中国中药杂志

2015, v.40(04) 661-666

[打印本页] [关闭]
本期目录(Current Issue) | 过刊浏览(Past Issue) | 高级检索(Advanced Search)

从蛋白质自组装的角度探析甘草附子配伍减毒机制
Investigating mechanism of toxicity reduction by combination of Glycyrrhizae Radix et Rhizoma and Aconiti Lateralis Radix Preparata on terms of proteins self-assembly

李冰洁;沈勇;廖日滔;高观祯;柯李晶;周建武;饶平凡;
LI Bing-jie;SHEN Yong;LIAO Ri-tao;GAO Guan-zhen;KE Li-jing;ZHOU Jian-wu;RAO Ping-fan;Institute of Biotechnology,Fuzhou University;Shanghai Institutes for Biological Sciences of Chinese Academy of Sciences-Zhejiang Gongshang University Joint Centre for Food and Nutrition Research;

摘要(Abstract):

甘草与附子配伍有减毒增效的作用。该研究从中药汤剂甘草和附子混煎过程中蛋白质自组装的现象入手,以甘草蛋白和附子主要毒性物质——乌头碱为研究对象,探析甘草附子配伍减毒机制。研究发现经分离纯化后的甘草蛋白在p H 5时可通过自组装形成粒径为(206.2±2.02)nm的稳定颗粒,且可与乌头碱形成平均粒径为(238.20±1.23)nm的甘草蛋白-乌头碱稳定颗粒。通过小鼠急性毒性实验发现注射乌头碱单体的小鼠全部死亡,而注射相同乌头碱含量的甘草蛋白-乌头碱颗粒的小鼠全部存活。对甘草蛋白-乌头碱胶体颗粒的稳定性的调查显示,该胶体颗粒在室温下较稳定,具有成为候选药物载体的可能性。综上所述,甘草蛋白可通过与乌头碱自组装而达到减毒效果。
The combination of Glycyrrhizae Radix et Rhizoma and Aconiti Lateralis Radix Preparata can increase efficacy and decrease toxicity. This study started from the phenomena of protein self-assembly in the mixed decoction of Glycyrrhizae Radix et Rhizoma with Aconiti Lateralis Radix Preparata. The attenuated mechanism was explored between the combination of Glycyrrhizae Radix et Rhizoma and Aconiti Lateralis Radix Preparata by using the protein of Glycyrrhizae Radix et Rhizoma and aconitine which was the major toxic component of Aconiti Lateralis Radix Preparata. Glycyrrhizae Radix et Rhizoma protein with aconitine could form stable particles which particle mean diameter was( 206. 2 ± 2. 02) nm and( 238. 20 ± 1. 23) nm at p H 5. 0 in normal temperature. Through the mouse acute toxicity experiment found that injection of aconitine monomer all mice were killed,and injection of Glycyrrhizae Radix et Rhizoma protein- aconitine particles with the same content of aconitine all mice survived. Survey the stability of Glycyrrhizae Radix et Rhizoma protein-aconitine shows that the colloid particles is stable at room temperature,and it has the possibility to candidate drug carrier. Glycyrrhizae Radix et Rhizoma protein can reduce the toxicity of aconitine through self-assembly.

关键词(KeyWords): 甘草蛋白;乌头碱;自组装;减毒
Glycyrrhizae Radix et Rhizoma protein;aconitine;self-assembly;toxicity reduction

Abstract:

Keywords:

基金项目(Foundation): 国家重点基础研究发展计划(973)项目(2010CB530605);; 福建省自然科学基金项目(2012J01133)

作者(Author): 李冰洁;沈勇;廖日滔;高观祯;柯李晶;周建武;饶平凡;
LI Bing-jie;SHEN Yong;LIAO Ri-tao;GAO Guan-zhen;KE Li-jing;ZHOU Jian-wu;RAO Ping-fan;Institute of Biotechnology,Fuzhou University;Shanghai Institutes for Biological Sciences of Chinese Academy of Sciences-Zhejiang Gongshang University Joint Centre for Food and Nutrition Research;

Email:

DOI:

参考文献(References):

扩展功能
本文信息
服务与反馈
本文关键词相关文章
本文作者相关文章
中国知网
分享