中国中药杂志

2009, v.34(03) 324-327

[打印本页] [关闭]
本期目录(Current Issue) | 过刊浏览(Past Issue) | 高级检索(Advanced Search)

人参总皂苷合黄连小檗碱对慢性心衰大鼠血浆BNP、心肌细胞内钙离子浓度的影响
Effects of ginseng total saponins with berberine on plasma brain natriuretic peptide and Ca~(2+) concentration in experimental rats with chronic congestive heart failure

李永民;陈晓春;刘华;罗飞;李刚;
LI Yongmin,CHEN Xiaochun,LIU Hua,LUO Fei,LI Gang(Department of Traditional Chinese Medicine,Hebei North University,Zhangjiakou 075029,China)

摘要(Abstract):

目的:研究人参总皂苷(ginseng total saponins,GS)合黄连小檗碱(berberine,Ber)对慢性心衰大鼠血流动力学、血浆脑钠肽(BNP)、心肌细胞内钙离子浓度[Ca2+]i的影响。方法:连续12 d皮下注射较大量异丙肾上腺素(ISO)制备慢性心衰大鼠模型,ISO总量80 mg.kg-1,随机分为4组:模型组,每天蒸馏水灌胃;GS+Ber组(各20 mg.kg-1)、卡托普利组(45 mg.kg-1),每天灌胃1次;参麦注射液组(5 mL.kg-1),每天腹腔注射1次。另设正常组,每天蒸馏水灌胃。连续给药4周,测定各组大鼠血流动力学变化、血浆BNP含量,流式细胞仪测定心肌细胞内[Ca2+]i。结果:与正常组相比,模型组左心室内压峰值(LVSP)、左心室等容收缩期压力上升最大速率(+dp/dtmax)、左心室舒张期压力下降最大速率(–dp/dtmax)明显下降,左心室舒张末压(LVEDP)、血浆BNP、心肌细胞内[Ca2+]i显著升高,均有显著性差异(P<0.01);与模型组相比,GS+Ber组LVSP,±dp/dtmax明显升高,LVEDP、血浆BNP、心肌细胞内[Ca2+]i显著降低,均有显著性差异(P<0.01,P<0.05),治疗效果接近卡托普利组,优于参麦注射液组。结论:GS+Ber对慢性心衰大鼠血流动力学异常、血浆BNP、心肌细胞内钙超载有良好改善作用。
Objective:To study the effects of total saponins of Panax ginseng combined with berberine on hemodynamics,Plasma brain natriuretic peptide and Ca2+ concentration in cardiac cells of chronic congestive heart failure rats.Method:Animal models of heart failure were induced by subcutaneously injecting a large dose of ISO(80 mg.kg-1)for 12 days Rat and then were randomly divided into four groups:model group was given distilled water by gavage;total saponins of P.ginseng combined with berberine group was treated with total saponins of P.ginseng and berberine(each 20 mg.kg-1;once per day)taken orally;captopril group was given captopril(45 mg.kg-1;once per day) orally;shenmai injection group was injected intraperitoneally with shenmai injection(5 mL.kg-1,once per day).The normal control group was given distilled water by gavage.After 4 weeks of drug treatment,rat To determine the contents of hemodynamics and plasma brain natriuretic peptide were determined and rat Ca2+ concentration in cardiomyocytes was measured by flow cytometer of each group.Result:Compared with normal control group,in model group can decrease the contents of LVSP,+dp/dtmax,and-dp/dtmax were decreased,but increase the contents of LVEDP,plasma brain natriuretic peptide and Ca2+ concentration were increased.The difference between two groups was significant meaning(P<0.01).Compared with model group,in total saponins of P.ginseng combined with berberine group can increase the contents of LVSP,+dp/dtmax,-dp/dtmax were increased,but were increased contents of LVEDP,plasma brain natriuretic peptide and Ca2+ concentration were decreased.The difference between two groups was significant was(P <0.01,P <0.05).Moreover,Total saponins of P.ginseng combined with berberine group had an efficacy similar to that of captopril group but superior to that of shenmai injection group.Conclusion:Total saponins of P.ginseng combined with berberine has a good effect on hemodynamics,plasma brain natriuretic peptide concentration and calcium overload in experimental rats with chronic congestive heart failure.

关键词(KeyWords): 人参总皂苷;黄连小檗碱;心力衰竭;血流动力学;脑钠肽;钙离子
ginseng total saponins;berberine;congestive heart failure;hemodynamics;brain natriuretic peptide;calciumion

Abstract:

Keywords:

基金项目(Foundation): 河北省科技支撑计划课题(08276101D-65)

作者(Author): 李永民;陈晓春;刘华;罗飞;李刚;
LI Yongmin,CHEN Xiaochun,LIU Hua,LUO Fei,LI Gang(Department of Traditional Chinese Medicine,Hebei North University,Zhangjiakou 075029,China)

Email:

DOI:

扩展功能
本文信息
服务与反馈
本文关键词相关文章
本文作者相关文章
中国知网
分享