中国中药杂志

2011, v.36(06) 729-733

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赤芍与白芍HPLC特征指纹谱的建立及其在质量控制中的应用
Characteristic chromatographic profile of Paeoniae Radix and its application in quality control of crude and processed drugs of different origins and processing methods

王瑞;黄山君;杨琪伟;石燕红;王峥涛;
WANG Rui1,2,HUANG Shanjun1,YANG Qiwei1,SHI Yanhong1,WANG Zhengtao1,2,3*(1.School of pharmacy,Shanghai University of Traditional Chinese Medicines,Shanghai 201203,China; 2.Shanghai R&D Center for Standardization of Traditional Chinese Medicines,Shanghai 201203,China; 3.MOE Key Laboratory for Standardization of Chinese Medicines,Institute of Chinese Materia Medica,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China)

摘要(Abstract):

目的:建立赤芍与白芍的高效液相色谱特征指纹分析方法,为科学评价和有效控制其质量提供方法。方法:采用Polaris C18-A色谱柱;流动相乙腈-磷酸水溶液(pH 3.0),梯度洗脱;检测波长230 nm;流速1.0 mL.min-1;测定了67批不同来源的赤芍与白芍样品的HPLC特征指纹谱。结果:指认了9个芍药苷类和酚酸类成分,并以龙胆苦苷为内标对特征指纹峰进行半定量分析,根据系统聚类分析和相似度分析结果,对赤芍与白芍进行了评价。结论:特征指纹谱可对赤芍和白芍进行鉴别,并能够用于赤芍和白芍的质量评价。
Objective: To establish a HPLC method of characteristic chromatographic profile for the quality control of Paeoniae Radix.Method: The 67 batches of samples were analyzed on a Polaris C18-A column with a gradient elution of acetonitrile and phosphate solution(pH 3.0) at a flow rate of 1.0 mL·min-1 and detected at 230 nm.Result: Nine main marker peaks were identified and semi-quantificated.By the similarity evaluation software for chromatographic fingerprint of traditional Chinese medicine(Version 2004A) and hierarchical clustering analysis,67 batches of Paeoniae Radix were classified.Conclusion: The method can be applied for quality assessment of Paeoniae Radix.

关键词(KeyWords): 赤芍;白芍;特征指纹谱;高效液相色谱法
Paeoniae Radix;characteristic chromatographic profile;HPLC

Abstract:

Keywords:

基金项目(Foundation): 国家“重大新药创制”科技重大专项(2009ZX09308-003,2009ZX09052-021);; 上海市自然科学基金项目(008ZR1419100);; 中医药行业科技专项(200707009)

作者(Author): 王瑞;黄山君;杨琪伟;石燕红;王峥涛;
WANG Rui1,2,HUANG Shanjun1,YANG Qiwei1,SHI Yanhong1,WANG Zhengtao1,2,3*(1.School of pharmacy,Shanghai University of Traditional Chinese Medicines,Shanghai 201203,China; 2.Shanghai R&D Center for Standardization of Traditional Chinese Medicines,Shanghai 201203,China; 3.MOE Key Laboratory for Standardization of Chinese Medicines,Institute of Chinese Materia Medica,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China)

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