中国中药杂志

2008, (11) 1291-1294

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超滤法测定甲基原薯蓣皂苷的血浆蛋白结合率
Determination of plasma protein binding rate of methyl protodioscin with ultrafiltration

姚志红;曹秀珍;邵萌;潘宇明;叶文才;姚新生;
YAO Zhi-hong1,CAO Xiu-zhen2,SHAO Meng3,PAN Yu-ming1,YE Wen-cai1,3,YAO Xin-sheng1,2(1.Institute of Traditional Chinese Medicine & Natural Products,Jinan University,Guangzhou 510632,China;2.Department of Natural Products Chemistry,Shenyang Pharmaceutical University,Shenyang 110016,China;3.College of Traditional Chinese Medicine,China Pharmaceutical University,Nanjing 210009,China)

摘要(Abstract):

目的:测定甲基原薯蓣皂苷的血浆蛋白结合率。方法:采用超滤法结合高效液相色谱-串联质谱联用法(HPLC-MS-MS)对甲基原薯蓣皂苷与大鼠血浆以及健康人血浆的蛋白结合率进行测定。结果:甲基原薯蓣皂苷与大鼠血浆在20.0,100,200μg.mL-1下的血浆蛋白结合率分别为(94.6±0.16)%,(91.6±0.35)%,(86.10±0.60)%。甲基原薯蓣皂苷在以上3个浓度下与健康人血浆的血浆蛋白结合率分别为(82.11±5.12)%,(84.54±0.32)%,(88.52±1.02)%。结论:甲基原薯蓣皂苷与血浆蛋白具有较强的结合。
Objective:To study the plasma protein binding rate of methyl protodioscin.Method: The ultrafiltration was employed to determine the plasma protein binding rate of methyl protodioscin.The plasma concentrations of methyl protodioscin were measured by HPLC-MS-MS.Result:The plasma protein binding rate of methyl protodioscin with rat plasma at the concentration of 20.0,100 and 200 μg·mL-1 were(94.6±0.16)%,(91.6±0.35)% and(86.10±0.60)%,respectively,while the plasma protein binding rate of methyl protodioscin with normal human plasma at the above concentrations were(82.11±5.12)%,(84.54±0.32)% and(88.52±1.02)%,respectively.Conclusion: The binding rate of methyl protodioscin with plasma protein is high.

关键词(KeyWords): 甲基原薯蓣皂苷;血浆蛋白结合率;超滤法;HPLC-MS-MS
methyl protodioscin;plasma protein binding rate;ultrafiltration;HPLC-MS-MS

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基金项目(Foundation): 国家自然科学基金项目(30300432)

作者(Author): 姚志红;曹秀珍;邵萌;潘宇明;叶文才;姚新生;
YAO Zhi-hong1,CAO Xiu-zhen2,SHAO Meng3,PAN Yu-ming1,YE Wen-cai1,3,YAO Xin-sheng1,2(1.Institute of Traditional Chinese Medicine & Natural Products,Jinan University,Guangzhou 510632,China;2.Department of Natural Products Chemistry,Shenyang Pharmaceutical University,Shenyang 110016,China;3.College of Traditional Chinese Medicine,China Pharmaceutical University,Nanjing 210009,China)

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