中国中药杂志

2020, v.45(20) 4929-4937

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杯鞘石斛中联苄类化学成分研究
Study on chemical bibenzyls in Dendrobium gratiosissimum

孙佳玮;刘继梅;陈日道;刘雨雨;李燕;岑山;陈晓梅;郭顺星;戴均贵;
SUN Jia-wei;LIU Ji-mei;CHEN Ri-dao;LIU Yu-yu;LI Yan;CEN Shan;CHEN Xiao-mei;GUO Shun-xing;DAI Jun-gui;State Key Laboratory of Bioactive Substance and Function of Natural Medicines,Key Laboratory of Natural Drug Biosynthesis of National Health Committee,Key Laboratory of Enzymes and Natural Drug Biocatalysis of Chinese Academy of Medical Sciences,Institute of Materia Medica,Chinese Academy of Medical Sciences & Peking Union Medical College;Department of Immunology,Institute of Medicinal Biotechnol

摘要(Abstract):

通过多种色谱手段从杯鞘石斛Dendrobium gratiosissimum乙醇提取物分离得到了19个化合物,运用波谱学方法分别鉴定为杯鞘石斛酚A(1),4,4′-二羟基-3,5-二甲氧基联苄(2),3,4-二羟基-5,3′,4′-三甲氧基联苄(3),4-羟基-3,5,4′-三甲氧基联苄(4),鼓槌石斛素(5),3,4′-二羟基-4,5,3′-三甲氧基联苄(6),3,4,4′-三羟基-5,3′-二甲氧基联苄(7),3-羟基-5,3′,4′-三甲氧基联苄(8),4′-羟基-3,5,3′-三甲氧基联苄(9),3,5,3′,4′-四甲氧基联苄(10),3,3′-二羟基-5-甲氧基联苄(11),杓唇石斛素(12),3,4-二羟基-5,4′-二甲氧基联苄(13),石斛酚(14),3,4′-二羟基-5-甲氧基联苄(15),2,6-二羟基-4-甲氧基-9,10-去氢菲(16),4,5-二羟基-2,6-二甲氧基-9,10-二氢菲(17),3,4-二羟基-5,4′-二甲氧基二苯乙烯(18)和4-羟基-3,5,4′-三甲氧基二苯乙烯(19)。其中化合物1为新化合物,化合物2~10,16,18和19为首次从该植物中分离得到。体外药理活性测定结果表明:化合物4对人肝癌细胞株HepG2有一定的抑制作用(IC_(50)为10.15μmol·L(-1));化合物7和12对HIV病毒具有中等强度的抑制作用,IC_(50)分别为9.35,9.15μmol·L(-1));化合物7和12对HIV病毒具有中等强度的抑制作用,IC_(50)分别为9.35,9.15μmol·L(-1);化合物10对IAV病毒具有中等强度的抑制作用,IC_(50)为8.90μmol·L(-1);化合物10对IAV病毒具有中等强度的抑制作用,IC_(50)为8.90μmol·L(-1)
Nineteen compounds were isolated and structurally characterized from an ethanol extract of Dendrobium gratiossimum, including dendrogratiol A(1), DDB-1(2), 3,4-dihydroxyl-5,3′,4′-trimethoxybibenzyl(3), amoenylin(4), chrysotoxine(5), DTB(6), 3,4,4′-trihydroxyl-5,3′-dimethoxybenzyl(7), 3-methylgiga(8), aloifol(9), gigantol tetramethyl ether(10), batatasin Ⅲ(11), moscatilin(12), moniliformine(13), gigantol(14), DMB(15), flavanthrinin(16), cannithrene-2(17), 3,4-dihydroxyl-5,4′-dimethoxystilbene(18) and 4-hydroxy-3,5,4′-trimethoxystilbene(19). 1 was a new compound, and 2-10, 16, 18 and 19 were obtained from this plant species for the first time. In vitro cytotoxic and antiviral activities of these isolates were evaluated, which displayed that 4 showed moderate cytotoxicity against human hepatoma cell line HepG2 with the IC_(50) of 10.15 μmol·L(-1)。
Nineteen compounds were isolated and structurally characterized from an ethanol extract of Dendrobium gratiossimum, including dendrogratiol A(1), DDB-1(2), 3,4-dihydroxyl-5,3′,4′-trimethoxybibenzyl(3), amoenylin(4), chrysotoxine(5), DTB(6), 3,4,4′-trihydroxyl-5,3′-dimethoxybenzyl(7), 3-methylgiga(8), aloifol(9), gigantol tetramethyl ether(10), batatasin Ⅲ(11), moscatilin(12), moniliformine(13), gigantol(14), DMB(15), flavanthrinin(16), cannithrene-2(17), 3,4-dihydroxyl-5,4′-dimethoxystilbene(18) and 4-hydroxy-3,5,4′-trimethoxystilbene(19). 1 was a new compound, and 2-10, 16, 18 and 19 were obtained from this plant species for the first time. In vitro cytotoxic and antiviral activities of these isolates were evaluated, which displayed that 4 showed moderate cytotoxicity against human hepatoma cell line HepG2 with the IC_(50) of 10.15 μmol·L(-1); 7 and 12 exhibited moderate inhibitory activity towards HIV virus with the IC_(50) of 9.35 and 9.15 μmol·L(-1); 7 and 12 exhibited moderate inhibitory activity towards HIV virus with the IC_(50) of 9.35 and 9.15 μmol·L(-1), respectively; and 10 displayed inhibitory activity against IAV virus with the IC_(50) of 8.90 μmol·L(-1), respectively; and 10 displayed inhibitory activity against IAV virus with the IC_(50) of 8.90 μmol·L(-1).

关键词(KeyWords): 杯鞘石斛;联苄类化合物;杯鞘石斛酚A;细胞毒;抗病毒
Dendrobium gratiossimum;bibenzyls;dendrogratiol A;cytotoxicity;antiviral activity

Abstract:

Keywords:

基金项目(Foundation): 中国医学科学院医学与健康科技创新工程项目(CIFMS-2017-I2M-3-013)

作者(Authors): 孙佳玮;刘继梅;陈日道;刘雨雨;李燕;岑山;陈晓梅;郭顺星;戴均贵;
SUN Jia-wei;LIU Ji-mei;CHEN Ri-dao;LIU Yu-yu;LI Yan;CEN Shan;CHEN Xiao-mei;GUO Shun-xing;DAI Jun-gui;State Key Laboratory of Bioactive Substance and Function of Natural Medicines,Key Laboratory of Natural Drug Biosynthesis of National Health Committee,Key Laboratory of Enzymes and Natural Drug Biocatalysis of Chinese Academy of Medical Sciences,Institute of Materia Medica,Chinese Academy of Medical Sciences & Peking Union Medical College;Department of Immunology,Institute of Medicinal Biotechnol

DOI: 10.19540/j.cnki.cjcmm.20200523.201

参考文献(References):

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