中国中药杂志

2010, v.35(20) 2666-2668

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天山雪莲组培苗与原药材中芦丁及紫丁香苷含量的比较
Comparison of rutin and syringin content between tissue culturing seedlings and botanical drug of Saussurea involucrata

吕亚丽;王春兰;郭顺星;董海玲;
LV Yali1,2,WANG Chunlan1,GUO Shunxing1,DONG Hailing1(1.Institute of Medicinal Plant Development,Chinese Academy of Medicinal Sciences &Peking Union Medical College,Beijing 100193,China;2.Baotou Medical College of Inner Mongolia Science & Technology University,Baotou 014040,China)

摘要(Abstract):

目的:比较天山雪莲组培苗与原药材中芦丁及紫丁香苷含量的差异。方法:采用HPLC,Hydro-RP C18柱(4.6mm×250 mm,5μm),流动相为乙腈-水(5∶95),流速1 mL.m in-1,柱温25℃,检测波长220 nm。结果:天山雪莲组培苗含有多种与原药材相同的有效化学成分,其中紫丁香苷的含量比原药材提高了近4.35倍。结论:利用组织培养方法提高天山雪莲苗质量具有良好的开发前景。
Objective: To compare the rutin and syringin content in tissue culturing seedlings and in botanical drug of Saussurea involucrata.Method: The HPLC with Hydro-RP C18(4.6 mm×250 mm,5 μm) column was used,a mixture of acetonitrile-water(5∶95) was used as a mobile phase,with flow rate of 1 mL·min-1,column temperature at 25 ℃ and detection wavelength at 220 nm.Result: The effective constituents of tissue culturing seedlings were almost similar to the botanical drug.And syringin in tissue culturing seedlings was increased 4.35 times.Conclusion: It has a good prospect to acquire high-quality S.involucrata by tissue culturing seedlings.

关键词(KeyWords): 天山雪莲;组培苗;芦丁;紫丁香苷;高效液相色谱法
Saussurea involucrata;tissue culturing seedlings;rutin;syringin;HPLC

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基金项目(Foundation): 国家高技术研究发展计划(863)项目(2008AA09Z405)

作者(Author): 吕亚丽;王春兰;郭顺星;董海玲;
LV Yali1,2,WANG Chunlan1,GUO Shunxing1,DONG Hailing1(1.Institute of Medicinal Plant Development,Chinese Academy of Medicinal Sciences &Peking Union Medical College,Beijing 100193,China;2.Baotou Medical College of Inner Mongolia Science & Technology University,Baotou 014040,China)

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