中国中药杂志

2018, v.43(16) 3329-3334

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羚羊角与山羊角蛋白质类成分比较研究
Comparison on proteins of Saigae Tataricae Cornu and Caprae Hircus Cornu

刘睿;朱振华;吴佳;钱大玮;段金廒;
LIU Rui;ZHU Zhen-hua;WU Jia;QIAN Da-wei;DUAN Jin-ao;Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization,and National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine,Nanjing University of Chinese Medicine;Jiangsu Key Laboratory of Research and Development in Marine Bio-resource Pharmaceutics;School of Pharmacy,Nanjing University of Chinese Medicine;

摘要(Abstract):

应用SDS-PAGE与LC-MS/MS技术对羚羊角与山羊角中蛋白质类成分进行鉴定分析研究。采用胶内酶切制备羚羊角与山羊角酶解肽段,通过nano LC-LTQ/Orbitrap MS鉴定蛋白质类成分,从羚羊角中鉴定了101个蛋白质,山羊角中鉴定了140个蛋白质;羚羊角中共含有43个角蛋白(KRT)及角蛋白相关蛋白(KAP),占总蛋白数量的42.6%,山羊角中KRT与KAP共52个,占总蛋白数量的37.1%。羚羊角与山羊角比较结果表明,两者共性成分是以角蛋白为主的分子结构相关蛋白质,两者在蛋白质种类构成方面具有相似性。该文研究为羚羊角与山羊角系统物质基础研究提供测定方法与实验数据,对角类动物药的功效物质基础研究、质量标准研究等具有指导意义。
SDS-PAGE and LC-MS/MS were used to identify proteins in Saigae Tataricae Cornu( SAH) and Caprae Hircus Cornu( GH). Trypsin digestion peptides from SAH and GH were obtained by in-gel digestion,after which nano LC-LTQ/Orbitrap MS was used to identify the proteins in SAH and GH. As a result,in total 101 proteins and 140 proteins were identified form SAH and GH,respectively. There were 43 keratins( KRTs) and keratin-associated proteins( KAPs) identified,which account for 42. 6% of the 101 proteins in SAH. The proportion of KRTs and KAPs in GH was 37. 1%. The comparison between SAH and GH showed that the main common components in SAH and GH were structural molecule activity proteins,such as KRTs and KAPs. In the present study,we provide determination method and experimental data for investigating the material basis of SAH and GH,guiding the investigation on effective material basis and quality standard of animal horn derived TCMs.

关键词(KeyWords): 羚羊角;山羊角;蛋白质;比较;LC-MS/MS
Saigae Tataricae Cornu;Caprae Hircus Cornu;proteins;comparison;LC-MS/MS

Abstract:

Keywords:

基金项目(Foundation): 江苏高校优势学科建设工程项目(PAPD);; 江苏省高校“青蓝工程”优秀青年骨干教师(2016年)项目;; 江苏省“333工程”第三层次(2016年)项目

作者(Author): 刘睿;朱振华;吴佳;钱大玮;段金廒;
LIU Rui;ZHU Zhen-hua;WU Jia;QIAN Da-wei;DUAN Jin-ao;Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization,and National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine,Nanjing University of Chinese Medicine;Jiangsu Key Laboratory of Research and Development in Marine Bio-resource Pharmaceutics;School of Pharmacy,Nanjing University of Chinese Medicine;

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DOI: 10.19540/j.cnki.cjcmm.20180511.001

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