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2020, v.45(19) 4643-4651

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不同栽培品种及干燥工艺白芍花中9种有效成分含量测定及主成分分析
Content determination and principal component analysis of nine active components in Paeonia lactiflora flowers from different cultivars and drying methods

谢凯莉;马喆;龚慕辛;苟雯婧;贺蕊;高慧敏;王智民;
XIE Kai-li;MA Zhe;GONG Mu-xin;GOU Wen-jing;HE Rui;GAO Hui-min;WANG Zhi-min;School of Traditional Chinese Medicine, Capital Medical University;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences;

摘要(Abstract):

建立UPLC-DAD同时测定不同栽培品种、处理方式白芍花瓣中的酚酸类、花色苷类、单萜苷类和黄酮类共9个有效成分的含量,采用Waters ACQUITY UPLC HSS T3 C_(18)色谱柱(2.1 mm×100 mm,1.8μm),以0.5%甲酸水溶液(A)-乙腈(B)为流动相进行梯度洗脱,流速为0.3 mL·min~(-1),检测波长采用切换波长法,柱温20℃,进样量2μL,得到不同来源和处理方式的白芍花样品中的上述成分含量。采用SIMCA 14.0软件对测定结果进行主成分分析,显示白芍花按照品种归为2类,亳州芍药为1类,白花川芍药和红花川芍药为1类,亳州芍药为上述成分综合含量高的品种;干白芍花按照烘干、晒干和阴干3种干燥方式分为3类,干燥方式以烘干为好;而产地、海拔、生长年限和开放程度对白芍花上述成分影响不大。含量测定结果可用于指导白芍花品种选择、采收和加工。
This study aims to establish an UPLC-DAD method for the simultaneous determination of nine active components in phenolic acids, anthocyanins, monoterpene glucosides and flavonoids in the petals of Paeonia lactiflora flowers from different cultivars and processing methods. UPLC analysis was performed on a Waters ACQUITY UPLC HSS T3 C_(18) column(2.1 mm × 100 mm, 1.8 μm)for gradient elution with a mobile phase consisting of 0.5% formic acid solution(A)-acetonitrile(B)at a flow rate of 0.3 mL·min~(-1).The detection wavelength was determined with a switched wavelength method and the column temperature maintained at 20 ℃, with an injection volume of 2 μL.The contents of the above components in the samples with different sources and treatment methods were obtained. The principal component analysis(PCA)of P. lactiflora flowers was conducted by using SIMCA 14.0 software, and the results showed that the P. lactiflora flowers could be classified into two categories according to cultivars, including P. lactiflora ′Bozhou-shaoyao′ as one category with high contents of the above components, P. lactiflora ′Baihuachuanshaoyao′ and P. lactiflora ′Honghuachuanshaoyao′ as the other category. Dried P. lactiflora flowers could be classified into three categories, including baking-drying, sun-drying and shade-drying, with baking-drying method as the optimal one.The P. lactiflora flowers were not greatly affected by origins, growing altitudes, growing years, or blooming stages.The results of contents determination can be used to guide the cultivar selection, harvest and processing of P. lactiflora flowers.

关键词(KeyWords): 白芍花;超高效液相色谱法;含量测定;主成分分析
Paeonia lactiflora flower;UPLC;content determination;principal component analysis

Abstract:

Keywords:

基金项目(Foundation): 国家重点研发计划项目(2017YFC1701903-3)

作者(Author): 谢凯莉;马喆;龚慕辛;苟雯婧;贺蕊;高慧敏;王智民;
XIE Kai-li;MA Zhe;GONG Mu-xin;GOU Wen-jing;HE Rui;GAO Hui-min;WANG Zhi-min;School of Traditional Chinese Medicine, Capital Medical University;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences;

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DOI: 10.19540/j.cnki.cjcmm.20200725.301

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