中国中药杂志

2016, v.41(11) 2112-2117

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广藿香内生真菌Diaporthe longicolla A616细胞毒活性次级代谢产物研究
Study on cytotoxic secondary metabolites of endophytic fungus Diaporthe longicolla A616 from Pogostemon cablin

王沫;陈玉婵;孙章华;谭国慧;李浩华;刘洪新;严寒静;郭晓玲;章卫民;
WANG Mo;CHEN Yu-chan;SUN Zhang-hua;TAN Guo-hui;LI Hao-hua;LIU Hong-xin;YAN Han-jing;GUO Xiao-ling;ZHANG Wei-min;State Key Laboratory of Applied Microbiology Southern China,Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application,Guangdong Open Laboratory of Applied Microbiology,Guangdong Institute of Microbiology;Guangdong Pharmaceutical University;

摘要(Abstract):

为了研究广藿香内生真菌Diaporthe longicolla A616的活性次级代谢产物。采用正相硅胶、C18反相、Sephadex LH-20凝胶、HPLC等色谱技术从该菌株的发酵产物中分离得到了10个化合物,经波谱数据分析和文献比对,分别鉴定为1,3-diamino-1,3-dimethylurea(1),(7R,9R)-7-hydroxy-9-propyl-5-nonen-9-olide(2),ergosta-5,7,22-trien-3β-ol(3),(22E,24R)-ergosta-4,6,8(14)-22-tetraen-3-one(4),(22E,24R)-3β,5α-dihydroxy-6β-ergosta-7,22-diene(5),citreoisocoumarin(6),glycerol monolinoleate(7),1-(2-hydroxyethoxy)ethyl(E)-octadec-9-enoate(8),cyclo-(L-Pro-L-Ala)(9),cyclo(L)-Pro-(L)-Val(10),其中,化合物6~10为首次从Diaporthe属真菌中分离得到。体外细胞毒活性结果显示化合物4和5对肿瘤细胞株SF-268,MCF-7,NCIH460和Hep G-2具有显著的抑制活性,IC50分别为5.3,6.5,12.2,6.1μmol·L-1和8.2,5.2,6.1,9.4μmol·L-1。
To study active secondary metabolites of endophytic fungus Diaporthe longicolla A616 isolated from Pogostemon cablin.Ten compounds were isolated from fermentation product of the strain 616 by silica gel,reverse phase silica gel,Sephadex-LH20,HPLC and so on. Their structures were identified as 1,3-diamino-1,3-dimethylurea( 1),( 7R,9R)-7-hydroxy-9-propyl-5-nonen-9-olide( 2),Ergosta-5,7,22-trien-3β-ol( 3),( 22 E,24R)-ergosta-4,6,8( 14)-22-tetraen-3-one( 4),( 22 E,24R)-3β,5α-dihydroxy-6β-ergosta-7,22-diene( 5),citreoisocoumarin( 6),glycerol monolinoleate( 7),1-( 2-hydroxyethoxy) ethyl( E)-octadec-9-enoate( 8),cyclo-( L-Pro-LAla)( 9),cyclo( L)-Pro-( L)-Val( 10),respectively,based on extensive spectroscopic analysis and literature comparisons. Compounds 6-10 were isolated from the genus Diaporthe for the first time. All isolated compounds were evaluated for in vitro cytotoxic activities against SF-268,MCF-7,NCI-H460 and Hep G-2 tumor cell lines. Compounds 4 and 5 showed potent growth inhibitory activities against the four cell lines with IC50 values of 5. 3,6. 5,12. 2,6. 1μmol·L-1and 8. 2,5. 2,6. 1,9. 4μmol·L-1,respectively.

关键词(KeyWords): Diaporthe longicolla;内生真菌;广藿香;细胞毒活性
Diaporthe longicolla;endophytic fungus;Pogostemon cablin;cytotoxic activity

Abstract:

Keywords:

基金项目(Foundation): 国家973前期专项(2014CB460613);; 广东省自然科学基金项目(2015A030313710);; 广东省科技计划项目(2015A030302060,2014A030304050)

作者(Author): 王沫;陈玉婵;孙章华;谭国慧;李浩华;刘洪新;严寒静;郭晓玲;章卫民;
WANG Mo;CHEN Yu-chan;SUN Zhang-hua;TAN Guo-hui;LI Hao-hua;LIU Hong-xin;YAN Han-jing;GUO Xiao-ling;ZHANG Wei-min;State Key Laboratory of Applied Microbiology Southern China,Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application,Guangdong Open Laboratory of Applied Microbiology,Guangdong Institute of Microbiology;Guangdong Pharmaceutical University;

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