中国中药杂志

2006, (13) 1083-1086

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四物汤对人骨髓基质细胞蛋白质表达的作用
Effects of Siwu Tang on protein expression of HFCL

郭平;王升启;
GUO Ping~(1,2),WANG Sheng-qi~(1)(1.Institute of Radiation Medicine,Academy of Military Medical Sciences,Beijing 100850,China; 2.Shandong University of Chinese Medicine,Jinan 250014,China)

摘要(Abstract):

目的:考察四物汤对骨髓基质细胞HFCL增殖及蛋白质表达的影响,探讨四物汤补血作用的分子机制。方法:分别用MTT法、流式细胞术和蛋白质组学方法测定四物汤对HFCL增殖、细胞周期及蛋白质表达的影响。结果:四物汤能促进HFCL的增殖,提高其增殖指数,并使HFCL的12种蛋白质表达上调,5种下调。结论:四物汤能促进HFCL增殖,作用于多个蛋白质靶点,间接发挥补血作用。
Objective: To investigate the effects of Siwu Tang on HFCL proliferation and protein expression of bone marrow stromal cells and to discuss molecular mechanism of enriching blood functions of Siwu Tang.Method: MTT,flow cytometry and proteomics were used respectively to measure the effect of Siwu Tang on HFCL proliferation,cell cycle and protein expression.Result: Siwu Tang can promote HFCL proliferation,increase proliferative index,up-regulate 12 kinds of proteins and down-regulate 5 kinds of proteins of HFCL.Conclusion: Through promoting HFCL proliferation and acting on multiple protein targets,the blood enriching function of Siwu Tang was exerted indirectly.

关键词(KeyWords): 四物汤;骨髓基质细胞;细胞增殖;蛋白质组学
Siwu Tang;bone marrow stromal cells;cell proliferation;proteomics

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金资助项目(30271617);; 全军医药卫生科研基金(01Z019);; 北京市“248”重大创新工程项目(H010210220113)

作者(Authors): 郭平;王升启;
GUO Ping~(1,2),WANG Sheng-qi~(1)(1.Institute of Radiation Medicine,Academy of Military Medical Sciences,Beijing 100850,China; 2.Shandong University of Chinese Medicine,Jinan 250014,China)

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