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牛黄中游离胆汁酸的薄层色谱定量
HPTLC Densitometric Determination of Free Bile Acids in Bezoar

张启明,严克东,田颂九,李社花,程静
Zhang Qiming, Li Shehua, Cheng Jing, Yan Kedong Tian Songjiu (National Institute for the Control of Pharmaceutical and Biological Products, Beijing 100050)

摘要(Abstract):

用薄层扫描法测定了牛黄中胆酸、鹅去氧胆酸、去氧胆酸的含量。样品用甲醇提取,在国产高效硅胶板上用异辛烷-乙酸丁酯-冰乙酸(4:2:1)为展开剂展开,可同时测定样品中已知的所有游离胆汁酸的含量。
Cholic acid (CA), chenodeoxycholic acid (CDCA) and deoxycholic acid (DCA) which are extracted with CH_3OH from Bezoar can be separated on HPTLC silica gel plate (made in China) with isooctane-n-butyl acetate-acetic acid (4: 2: 1), and the three bile acids were determined by TLC densitometry.

关键词(KeyWords): 薄层扫描;牛黄;胆酸;鹅去氧胆酸;去氧胆酸
TLC-densitmetry; Bezoar; cholic acid; chenodeoxycholic acid; deoxycholic acid

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作者(Author): 张启明,严克东,田颂九,李社花,程静
Zhang Qiming, Li Shehua, Cheng Jing, Yan Kedong Tian Songjiu (National Institute for the Control of Pharmaceutical and Biological Products, Beijing 100050)

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