中国中药杂志

2020, v.45(01) 113-118

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UPLC-MS比较有机酸定向炮制黄芪中12种活性成分的含量
Comparison of 12 constituents of Astragali Radix directionally processed with organic acid by UPLC-MS

刘蓬蓬;单国顺;张凡;史辑;贾天柱;
LIU Peng-peng;SHAN Guo-shun;ZHANG Fan;SHI Ji;JIA Tian-zhu;School of Pharmacy, Liaoning University of Traditional Chinese Medicine;Key Laboratory of Processing Theory Analysis under State Administration of Traditional Chinese Medicine;Chinese Materia Medica Processing Engineering Center of Liaoning Province;

摘要(Abstract):

建立同时测定黄芪中4种皂苷类成分和8种黄酮类成分的UPLC-MS分析方法,分析有机酸定向炮制黄芪对苷及苷元成分含量的影响。采用ACQUITY UPLC HSS T_3色谱柱(2.1 mm×100 mm,1.8μm),0.1%甲酸水-0.1%甲酸乙腈溶液为流动相,梯度洗脱,流速0.5 mL·min~(-1),柱温30℃,检测波长260 nm,进样量2μL。采用电喷雾离子源(ESI~+),在正离子模式下进行监测扫描。12种成分在各自的浓度范围内均有良好的线性关系(R~2≥0.999 2),平均回收率均较好。结果表明黄芪经有机酸定向炮制后,皂苷类成分无显著变化,黄酮类成分既有量变又有质变。所建立的UPLC-MS方法可为有机酸定向炮制黄芪对苷及苷元成分含量影响的分析提供方法参考。
To establish an UPLC-MS method for the simultaneous content determination of 4 saponins and 8 flavonoids, in order to analyze the effect of Astragali Radix directionally processed with organic acid on the content of glycosides and aglycones. The separation was carried out on ACQUITY UPLC HSS T_3(2.1 mm×100 mm, 1.8 μm), the mobile phase was eluted with the mixture of 0.1 mol·L~(-1) formic acid water solution and 0.1 mol·L~(-1) formic acid acetonitril in a gradient mode. The detection wavelength was 260 nm, the flow rate was 0.5 mL·min~(-1), the column temperature was 30 ℃, and the injection volume was 2 μL. Mass spectrometry analysis was performed with an electrospray ionization(ESI) source in a positive ion mode. The 12 constituents showed good linear relations within their own ranges(R~2≥0.999 2),with good average recoveries. The results showed no significant change in saponins but both qualitative and quantitative changes in flavonoids after directional processing of Astragali Radix with organic acid. The established method can provide methodological reference for analyzing the effect of Astragali Radix directionally processed with organic acid on glycosides and aglycones.

关键词(KeyWords): 黄芪;超高效液相色谱-串联质谱法;皂苷;黄酮;含量测定;定向炮制
Astragali Radix;UPLC-MS;saponin;flavonoid;content determination;directional processing

Abstract:

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基金项目(Foundation): 国家中医药管理局中药炮制技术传承基地建设项目(2015132)

作者(Author): 刘蓬蓬;单国顺;张凡;史辑;贾天柱;
LIU Peng-peng;SHAN Guo-shun;ZHANG Fan;SHI Ji;JIA Tian-zhu;School of Pharmacy, Liaoning University of Traditional Chinese Medicine;Key Laboratory of Processing Theory Analysis under State Administration of Traditional Chinese Medicine;Chinese Materia Medica Processing Engineering Center of Liaoning Province;

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DOI: 10.19540/j.cnki.cjcmm.20191001.307

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