中国中药杂志

2020, v.45(16) 3837-3843

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百蕊草休眠解除前后种胚的转录组分析
Transcriptome analysis of seed embryo in dormancy and dormancy release state of Thesium chinense

张成才;高真;罗丽娜;张子璇;王将;卢富华;向增旭;
ZHANG Cheng-cai;GAO Zhen;LUO Li-na;ZHANG Zi-xuan;WANG Jiang;LU Fu-hua;XIANG Zeng-xu;College of Horticulture, Nanjing Agricultural University;Institute of Medicinal Plant Development,Peking Union Medical College, Chinese Academy of Medical Sciences;

摘要(Abstract):

利用外源GA_3打破百蕊草种子休眠,采用高通量测序技术对百蕊草休眠种胚和休眠解除种胚进行转录组测序并进行系统的生物信息学分析。结果表明,外源GA_3可有效打破百蕊草种子休眠;通过转录组测序得到上调差异基因73 794个,下调差异基因42 776个;共有116 570个差异基因被GO功能注释到代谢过程、细胞过程、细胞、细胞组分、结合和催化活性等GO条目。对26 508个差异表达基因进行Pathway显著性富集分析,共发现133个代谢通路,休眠前后DEGs主要富集在翻译、碳水化合物代谢、折叠、分类及降解和氨基酸代谢等途径,基于KEGG数据库中注释结果,共发现20条与百蕊草种子休眠解除相关的代谢通路。百蕊草种子休眠解除是一个复杂的生物学过程,包括细胞形态构建、次生代谢产物合成、糖代谢以及植物信号转导等,其中植物激素信号转导是调控休眠解除的关键因素之一。qRT-PCR验证结果显示测序结果和实际结果一致。
We used exogenous GA_3 to break the seed dormancy of Thesium chinense. We used high-throughput sequencing technology was used to sequence the transcriptome of dormant seed embryos and dormancy breaking seed embryos of Th. chinense, and the data was analyzed bioinformatically and systematically. The results showed that exogenous GA_3 could effectively break the seed dormancy of Th. chinense; 73 794 up-regulated genes and 42 776 down regulated genes were obtained by transcriptome sequencing; 116 570 diffe-rential genes were annotated by GO function to GO items such as metabolism process, cell process, cell, cell component, binding and catalytic activity. A total of 133 metabolic pathways were found by Pathway analysis of 26 508 differentially expressed genes. In the process of dormancy release, DEGs were mainly enriched in translation, carbohydrate metabolism, folding, classification, degradation and amino acid metabolism. Based on the annotation results in KEGG database, 20 metabolic pathways related to dormancy release were found. Dormancy release of Th. chinense seeds is a complex biological process, including cell morphology construction, secondary metabolite synthesis, sugar metabolism and plant signal transduction, among which plant hormone signal transduction is one of the key factors to regulate dormancy release. The results of qRT-PCR showed that the sequencing results were consistent with the actual results.

关键词(KeyWords): 百蕊草;休眠;外源GA_3;转录组;差异基因
Thesium chinense;dormancy;exogenous GA_3;transcriptome;differential genes

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基金项目(Foundation): 现代农业产业技术体系建设专项(CARS-21);; 中央高校基本科研业务费专项基金项目(Z52911218)

作者(Author): 张成才;高真;罗丽娜;张子璇;王将;卢富华;向增旭;
ZHANG Cheng-cai;GAO Zhen;LUO Li-na;ZHANG Zi-xuan;WANG Jiang;LU Fu-hua;XIANG Zeng-xu;College of Horticulture, Nanjing Agricultural University;Institute of Medicinal Plant Development,Peking Union Medical College, Chinese Academy of Medical Sciences;

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