中国中药杂志

2013, v.38(14) 2314-2317

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UPLC同时测定藿香正气口服液中甘草苷、柚皮芸香苷、橙皮苷、甘草酸铵、厚朴酚、和厚朴酚的含量
Simultaneous determination of six ingredients in Huoxiang Zhengqi oral liquid by UPLC

余佳文;邓开英;彭涛;朱必越;刘红亚;
YU Jia-wen1,DENG Kai-ying2,3,PENG Tao1,ZHU Bi-yue4,LIU Hong-ya5(1.Chongqing Fuling Pharmaceutical Co.,Ltd.,Chongqing 408000,China; 2.Chongqing Institute of Food and Drug for Control,Chongqing 401121,China; 3.Chongqing Engineering Research Center for Pharmaceutical Process and Quality Control,Chongqing 401121,China; 4.West China School of Pharmacy,Sichuan University,Chengdu 610041,China; 5.Sichuan Academy of Chinese Medicine Sciences,Chengdu 610041,China)

摘要(Abstract):

目的:建立同时测定藿香正气口服液中甘草苷、柚皮芸香苷、橙皮苷、甘草酸铵、厚朴酚以及和厚朴酚6个成分的UPLC分析方法。方法:采用Zorbax Eclipse C18柱,以乙腈(A)-0.05%磷酸溶液(B)为流动相,梯度洗脱(0~5 min,17%~20%A,5~12 min,20%~50%A,12~18 min,50%~70%A,18~18.5 min,70%~95%A,18.5~23.5 min,95%A),流速0.42mL.min-1,检测波长220 nm,柱温30℃。结果:甘草苷、柚皮芸香苷、橙皮苷、甘草酸铵、和厚朴酚以及厚朴酚进样量分别在0.001 7~0.034,0.003 4~0.068,0.006 4~0.128,0.012 8~0.256,0.003 2~0.064,0.006 4~0.128μg线性关系良好,平均加样回收率分别为103.3%,98.39%,98.29%,102.1%,98.45%,102.2%,RSD分别为2.1%,1.0%,1.5%,2.3%,0.90%,2.0%。结论:UPLC分离效果及重复性好,且快速简便,可作为藿香正气口服液的质量控制方法。
Objective: To develop a UPLC method for the simultaneous determination of liquiritin,narirutin,hesperidin,ammonium glycyrrhetate,honokiol and magnolol in Huoxiang Zhengqi oral liquid.Method: A Zorbax Eclipse C18 column was used with the mobile phase of acetonitrile and 0.05% phosphate acid by gradient elution at the detection wavelength of 220 nm.The flow rate was 0.42 mL· min-1 and the column temperature was 30 ℃.Result: The calibration curves were linear in the ranges of 0.001 7-0.034,0.003 4-0.068,0.006 4-0.128,0.012 8-0.256,0.003 2-0.064,0.006 4-0.128 μg,respectively.The average recoveries were 103.3%,98.39%,98.29%,102.1%,98.45%,102.2% with RSDs of 2.1%,1.0%,0.50%,2.3%,0.9%,2.0%,respectively.Conclusion: The UPLC method was simple,rapid and accurate,it could be used for quality control of Huoxiang Zhengqi oral liquid.

关键词(KeyWords): 藿香正气口服液;甘草苷;柚皮芸香苷;橙皮苷;甘草酸铵;和厚朴酚;厚朴酚;UPLC
Huoxiang Zhengqi oral liquid;liquiritin;narirutin;hesperidin;ammonium glycyrrhetate;honokiol;magnolol;UPLC

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作者(Author): 余佳文;邓开英;彭涛;朱必越;刘红亚;
YU Jia-wen1,DENG Kai-ying2,3,PENG Tao1,ZHU Bi-yue4,LIU Hong-ya5(1.Chongqing Fuling Pharmaceutical Co.,Ltd.,Chongqing 408000,China; 2.Chongqing Institute of Food and Drug for Control,Chongqing 401121,China; 3.Chongqing Engineering Research Center for Pharmaceutical Process and Quality Control,Chongqing 401121,China; 4.West China School of Pharmacy,Sichuan University,Chengdu 610041,China; 5.Sichuan Academy of Chinese Medicine Sciences,Chengdu 610041,China)

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