中国中药杂志

2019, v.44(10) 2046-2050

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产哈巴苷和哈巴俄苷玄参内生真菌的分离及鉴定
Isolation and identification of endophytic fungi producing harpagoside and harpagide from Scrophularia ningpoensis

沈湛云;朱波;张泉龙;秦路平;
SHEN Zhan-yun;ZHU Bo;ZHANG Quan-long;QIN Lu-ping;College of Pharmaceutical Sciences,Zhejiang Chinese Medical University;College of Traditional Chinese Medicine,Zhejiang Pharmaceutical College;

摘要(Abstract):

从玄参根、主茎、侧枝、叶片中分离得到内生真菌,采用高效液相和液质联用的方法对内生真菌的代谢产物进行分析,筛选能够产哈巴苷或哈巴俄苷的内生真菌,根据菌株的形态学特征,结合分子生物学的系统进化分析进行菌株鉴定。结果表明从玄参中共分离得到内生真菌210株,合并为46个形态型,分属于2纲、9目、13科、17属。HPLC初步筛选出2株内生真菌能够分别产哈巴苷和哈巴俄苷,LC-MS进一步验证得到了能产哈巴俄苷的目标菌株ZJ17,鉴定为Alternaria alternate;以及能产哈巴苷的目标菌株ZJ25,鉴定为A.gaisen。
The endophytic fungi from root,main stem,branch and leaf of Scrophularia ningpoensis were isolated from Zhejiang,whether these strains could yield harpagide or harpagoside were tested by HPLC and LC-MS. According to the morphological characteristic and the similarity of the nucleotide sequence of internal transcribed spacer( ITS) between r DNAs,the strains producing harpagide or harpagoside were identified. The results showed that 210 strains were isolated from the samples,which were classified into 9 orders,13 families and 17 genera by morphological study. Harpagide was detected in endogenous fungi ZJ17 and harpagoside was detected in endogenous fungi ZJ25 by HPLC coupled with LC-MS. ZJ17 was identified as Alternaria alternate and ZJ25 was identified as A.gaisen by its morphology and authenticated by ITS( ITS4 and ITS5 regions and the intervening 5. 8 S rDNA region).

关键词(KeyWords): 玄参;内生真菌;哈巴苷;哈巴俄苷
Scrophularia ningpoensis;endophytic fungi;harpagide;harpagoside

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(81673528);; 浙江省教育厅科研项目(Y201636676);浙江省教育厅访问工程师校企合作项目(FG2017006)

作者(Authors): 沈湛云;朱波;张泉龙;秦路平;
SHEN Zhan-yun;ZHU Bo;ZHANG Quan-long;QIN Lu-ping;College of Pharmaceutical Sciences,Zhejiang Chinese Medical University;College of Traditional Chinese Medicine,Zhejiang Pharmaceutical College;

DOI: 10.19540/j.cnki.cjcmm.20190301.008

参考文献(References):

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