中国中药杂志

2018, v.43(24) 4855-4861

[打印本页] [关闭]
本期目录(Current Issue) | 过刊浏览(Past Issue) | 高级检索(Advanced Search)

基于CRITIC权重赋值的Box-Behnken响应面法优化甘草黄酮纳米海绵制备工艺
Optimization of preparation procedures for nano-sponges of flavonoids from Glycyrrhizae Radix et Rhizoma by Box-Behnken response surface methodology based on CRITIC weighted evaluation

孙强;李小芳;罗开沛;赵甜甜;马祖兵;郑宇;谢龙;
SUN Qiang;LI Xiao-fang;LUO Kai-pei;ZHAO Tian-tian;MA Zu-bing;ZHENG Yu;XIE Long;Pharmacy College,Chengdu University of Traditional Chinese Medicine,The Ministry of Education Key Laboratory of Standardization of Chinese Herbal Medicine,Key Laboratory of Systematic Research,Development and Utilization of Chinese Medicine Resources in Sichuan Province—Key Laboratory Breeding Base of Co-founded by Sichuan Province and MOST;

摘要(Abstract):

采用搅拌-冷冻干燥法制备甘草黄酮纳米海绵(LF-NSP),在单因素基础上,以搅拌温度、搅拌时间以及搅拌速度为自变量,载药量、粒径以及总评"归一值"为响应值,采用CRITIC权重赋值结合Box-Behnken响应面法优化制备工艺。采用扫描电子显微镜(SEM),傅里叶变换红外光谱法(FT-IR)和差式扫描量热法(DSC)对纳米海绵的性质进行表征,并考察其体外释放行为。结果表明,甘草黄酮纳米海绵的最优制备工艺为药辅比1∶2,交联剂DPC与β-CD比例4∶1,搅拌温度45℃,搅拌时间4. 8 h,搅拌速度245 r·min-1。所制备甘草黄酮纳米海绵的综合评分为94. 78;傅里叶变换红外光谱和差式扫描量热均表明甘草黄酮纳米海绵的形成,扫描电子显微镜表明其形态为球形颗粒。体外释放实验中甘草黄酮纳米海绵240 min的累积释放度为81. 8%,而原料药仅为31. 5%;纳米海绵可以明显提高甘草黄酮的溶出度。
In this paper,nano-sponges of flavonoids from Glycyrrhizae Radix et Rhizoma( LF-NSP) were prepared by agitationfreeze drying method. Box-benhnken design and response surface method based on the single factor experiment was used to optimize the preparation process,with the stirring temperature as well as stirring time and speed as the independent variables,while with drug loading,particle size and the generalized " normalized value" as the response values. In addition,the nano-sponges were characterized by scanning electron microscope( SEM),infraredspectroscopy( FT-IR) and differential scanning calorimetry( DSC),and its release in vitro was also investigated. The results showed that the optimum preparation conditions for glycyrrhizin nano-sponges were as follows:The proportion of main drug and auxiliary drug was 1∶ 2; the proportion of crosslinking agent DPC and β-CD was 4∶ 1; stirring temperature 45 ℃ for 4. 8 h at 245 r·min-1. The comprehensive score of LF-NSP prepared under these conditions was 94. 78. FT-IR and DSC results indicated the formation of Glycyrrhiza flavonoids nano-sponges,and SEM showed that they were spherical particles in shape. In release experiment in vitro,the cumulative release of glycyrrhizin flavonoids nano-sponges for 240 min was 81. 8%,while that of crude drug was only 31. 5%. Nano-sponges can significantly improve the dissolution of flavonoids from Glycyrrhizae Radix et Rhizoma.

关键词(KeyWords): 甘草黄酮;纳米海绵;权重;制备工艺;Box-Behnken响应面法;体外释放度
flavonoids from Glycyrrhizae Radix et Rhizoma;nano-sponge;weight;preparation process;Box-Benhnken design and response surface method;in vitro release degree

Abstract:

Keywords:

基金项目(Foundation): 四川省中医药管理局科研项目(2018YY001);; 成都市科技局科技惠民项目(2016-HM01-00299-SF);; 成都中医药大学校基金项目(ZRQN1772)

作者(Author): 孙强;李小芳;罗开沛;赵甜甜;马祖兵;郑宇;谢龙;
SUN Qiang;LI Xiao-fang;LUO Kai-pei;ZHAO Tian-tian;MA Zu-bing;ZHENG Yu;XIE Long;Pharmacy College,Chengdu University of Traditional Chinese Medicine,The Ministry of Education Key Laboratory of Standardization of Chinese Herbal Medicine,Key Laboratory of Systematic Research,Development and Utilization of Chinese Medicine Resources in Sichuan Province—Key Laboratory Breeding Base of Co-founded by Sichuan Province and MOST;

Email:

DOI: 10.19540/j.cnki.cjcmm.20181105.008

参考文献(References):

扩展功能
本文信息
服务与反馈
本文关键词相关文章
本文作者相关文章
中国知网
分享