中国中药杂志

2011, v.36(07) 850-853

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地龙体外抗凝血部位的制备工艺研究
Study on best preparation procedure of Lumbricus for anticoagulated blood region in vitro

李娟;徐玉玲;王永香;王振中;萧伟;
LI Juan1,XU Yuling2,WANG Yongxiang2,3,WANG Zhenzhong2,3,XIAO Wei1,2(1.Nanjing University of Traditional Chinese Medicine,Nanjing 210046,China;2.Jangsu Kanion Pharmaceutical Co.,Ltd.,Lianyungang 222001,China;3.State Key Laboratory of Pharmaceutical New-tech for Chinese Medicine,Lianyungang 222001,China)

摘要(Abstract):

目的:采用体外活性评价法,研究地龙基于膜分离技术抗凝血部位的制备工艺。方法:以蛋白质含量和酶活性为指标,采用单因素考察和正交试验优选地龙的提取、精制、浓缩工艺。结果:地龙体外抗凝血部位的制备工艺为地龙粗粉加入15倍量0.9%NaC l溶液,37℃超声提取3次,每次40 m in;将上述提取液经30×103超滤膜进行精制,超滤液经10×103超滤膜进行浓缩。结论:超声提取技术与膜分离技术相结合,能够提高地龙抗凝血部位的分离纯化效果,有利于活性部位的进一步研究。
Objective: Applying for the activity of enzyme in vitro,the research optimized the best preparation procedure for the anticoagulated blood region from Lumbricus.Method: All through our experiment,the content of protein and theactivity of enzyme were examined.The extraction process,the refining technology,concentration processes of Lumbricus were optimized with single factor checking and orthogonal design method.Result: At 37 ℃,the coarse powder of Lumbricus soaking with 15 fold of 0.9% sodium chloride and ultrasonic extracting 40 minites for three times was the best ultrasonic extraction.Utrafiltration membrane with molecular weights of 30×103 for refining and 10×103 for concentrating were selected.Conclusion: Ultrasonic extraction and membrane separation technology,to well improve the effect of purification for the anticoagulant site of Lumbricus,is conducive to further study.

关键词(KeyWords): 地龙;超声提取法;超滤技术;纤维蛋白平板法;活性测定
Lumbricus;ultrasonic extraction;ultrafiltration technique;fibrin plate method;determination of activity

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基金项目(Foundation): 国家“重大新药创制”科技重大专项(2009ZX09313-032,2009ZX09308-003)

作者(Author): 李娟;徐玉玲;王永香;王振中;萧伟;
LI Juan1,XU Yuling2,WANG Yongxiang2,3,WANG Zhenzhong2,3,XIAO Wei1,2(1.Nanjing University of Traditional Chinese Medicine,Nanjing 210046,China;2.Jangsu Kanion Pharmaceutical Co.,Ltd.,Lianyungang 222001,China;3.State Key Laboratory of Pharmaceutical New-tech for Chinese Medicine,Lianyungang 222001,China)

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