中国中药杂志

2000, (04)

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积雪草的资源分布与生药鉴别
Comparison of ITS Sequences between Wild Ginseng DNA and Garden Ginseng DNA

陈瑶,秦路平,郑汉臣,张朝晖
MA Xiao jun 1,WANG Xiao quan 2,XIAO Pei gen 1,HONG De yuan 2 (1.Institute of Medicinal Plant Chinese Academy of Medical Sciences and Peking union Medical College, Beijing 100094, China; 2.Institute of Botany, Chinese Academy of Sciences, Beij

摘要(Abstract):

目的 :对国产积雪草的资源分布与形态组织学进行了研究 ,为开发利用该植物资源 ,制订药材质量标准提供依据。方法 :野外采集结合文献查阅与显微切片观察。结果 :列出了资源分布表。对药材的性状 ,茎、叶炳和叶的显微特征进行了描述、绘图。结论 :支持了Urban关于该属植物分类位置的观点
Objective: To compare the genetic differences between wild ginseng and garden ginseng ( Panax ginseng ) Method:The sequences of ITS 1 and ITS 2 of wild ginsengs were determined on LKB DNA sequencing station through Si lver Sequence TM DNA Sequencing System. The sequences were aligned with DNA SIS software. Results and conclusion: The ITS 1 and ITS 2 of Panax were 220~221 and 222~224 bases in length repectively. In Panax ginsehg, the seqences of ITS 1 were very stable, but ITS 2 were changeable. The ITS 2 sequences of No. 87 and No. 110 of the wild ginseng collected from Fusong Heilongjiang (China) were exactly the same as those of No. U41680(Jun Wen) and No. U41682(Jun Wen) of garden ginseng collected from Heilongjiang Province (China) and Korea respectively, but different from those of No. U41681(Jun Wen) from Hubei Province (China) in three bases (447,449,450) The result implies that the cultivated ginsengs may have been introduced from two different pupolations of the wild ginseng.

关键词(KeyWords): 积雪草;资源分布;显微鉴别
wild ginseng; cultivated ginseng; ITS; comparison

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基金项目(Foundation): 上海-联合利华研究与发展基金资助项目!(9802)

作者(Author): 陈瑶,秦路平,郑汉臣,张朝晖
MA Xiao jun 1,WANG Xiao quan 2,XIAO Pei gen 1,HONG De yuan 2 (1.Institute of Medicinal Plant Chinese Academy of Medical Sciences and Peking union Medical College, Beijing 100094, China; 2.Institute of Botany, Chinese Academy of Sciences, Beij

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