张红兵;韩梅;温进坤;
ZHANG Hongbing1,2,HAN Mei1*,WEN Jinkun1(1.Hebei Laboratory of Medical Biotechnology,Institute of Basic Medicine,Heibei Medical University,Shijiazhuang 050017,China;2.Biological Science and Engineering Institute,Hebei University of Economics and Business,Shijiazhuang 050061,China)

• 1:河北医科大学基础医学研究所河北省医学生物技术重点实验室
• 2:河北经贸大学生物科学与工程学院

摘要(Abstract)：

目的:观察欧亚旋覆花总黄酮类提取物(FE)对大鼠血管球囊损伤诱导的氧化应激反应的影响。方法:采用主动脉球囊剥脱后血管狭窄大鼠模型,将实验动物分成四组:对照组、模型组、FE组和阳性药物(卡托普利,CAP)组;采用常规HE染色法观察血管的形态学变化以确定最佳给药浓度并对比FE与CAP的疗效,分别采用硫代巴比妥酸(TBA)法测定大鼠血清中丙二醛(MDA)的含量,用黄嘌呤氧化酶法测定大鼠血清中超氧化物歧化酶(SOD)活力,用二氢乙锭(DHE)测定超氧阴离子(O2.-)的水平,用免疫组化和W estern b lot检测血管组织中的SOD表达变化。结果:FE的有效剂量为50 mg.kg-1.d。在此浓度时,FE可显著降低血管球囊损伤大鼠血清中MDA含量(P<0.01),提高血清和血管组织中SOD的活力(P<0.05),降低血管组织中O2.-水平。因此,FE对损伤诱导的血管内膜增生具有一定的抑制作用,达到与阳性对照药物相似的疗效。结论:从欧亚旋覆花中提取的FE可通过提高血管系统抗氧化活性而抑制损伤诱导的氧自由基生成,这可能是该药物抑制内皮剥脱诱导的血管内膜增生的机制之一。
Objective: To observe the inhibition of flavonoids extract(FE) from Inula britannica on oxidative stress in rat aorta after balloon injury.Method: The model of vascular intimal hyperplasia was established by balloon injury.The rats were randomly divided into four groups: control,model,FE and captopril(CAP,positive control).The FE group was treated by intragastric administration with FE in dose of 12.5,25,50 mg·kg-1·d-1.The level of malondialdehyde(MDA) and superoxide dismutase(SOD) in serum were detected by thiobarbituric acid(TAB) method and xanthine oxidase method respectively,and superoxide anion(O2-) in vessel was detected by dihydroethidium(DHE) staining.The histochemistry,immunohistochemistry and Western blot were used to observe the changes of appearance and SOD expression in vascular tissues after balloon injury.Result: The best concentration of FE to rats was 50 mg·kg-1·d-1.The neointima thickness in the model group was significantly higher than that in the FE group(50 mg·kg-1·d-1) and control group at 14 days after balloon injury(P<0.01).The lever of MDA in serum of FE group was decreased(P<0.01) and SOD was increased(P<0.05) in both serum and vascular tissues.The level of O-·2 in the drug group was lower than that in the model group.Conclusion: FE can enhance the antioxidation capacities of vessel tissues by suppressing the formation of O2-induced by injury,by which FE inhibites neointima formation after balloon injury in rat.

关键词(KeyWords)： 欧亚旋覆花;总黄酮类提取物;球囊剥脱损伤;氧化应激
Inula britannica;flavonoids;balloon injury;oxidative

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(30670845);; 河北省自然科学基金资助课题(C2007000831)

作者(Author): 张红兵;韩梅;温进坤;
ZHANG Hongbing1,2,HAN Mei1*,WEN Jinkun1(1.Hebei Laboratory of Medical Biotechnology,Institute of Basic Medicine,Heibei Medical University,Shijiazhuang 050017,China;2.Biological Science and Engineering Institute,Hebei University of Economics and Business,Shijiazhuang 050061,China)

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DOI:

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