中国中药杂志

2005, (19)

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不同填料的固相小柱对人参皂苷Re血浆样品萃取回收率的研究
Study on the extraction recovery of ginsenoside Re in plasma by different solid-phase cartridges

茅向军,章晨峰,孙棣,盛龙生,王广基,刘文英
MAO Xiang-jun,ZHANG Chen-feng,SUN Di,SHENG Long-sheng,WANG Guang-ji,LIU Wen-ying(China Pharmaceutical University,Nanjing 210009,China)

摘要(Abstract):

目的:以人参皂苷Re为研究对象,考察固相萃取小柱的洗脱条件和不同填料固相小柱的萃取效率,从而确定固相萃取人参皂苷Re血浆样品的实验方法。方法:以水,乙腈及不同含量的甲醇对固相小柱进行洗脱,将洗脱液在Zorbax SB-C18(4.6 mm×75 mm,5μm)柱上以乙腈-水进行梯度洗脱,进行高效液相色谱分析。确定人参皂苷Re在固相萃取小柱上的洗脱条件。通过比较不同固相填料的萃取回收率,选择最佳的固相萃取小柱。结果:最大可用甲醇清洗含量为40%甲醇,甲醇洗脱最小含量为80%的甲醇;在所考察的3种固相填料中,以Waters OasisHLB柱为固相填料时样品的萃取回收率最好。结论:经试验,Waters Oasis HLB柱的萃取回收率在103%~113%,适用于血浆中人参皂苷Re的分析。
Objective: To optimize the solid-phase extraction method by comparison of the extraction recovery of ginsenoside Re plasma samples.Method: After extracted by different solid-phase cartridges with water,acetonitrile,and different content methanol elution,the plasma samples were analyzed on an Zorbax SB-C_(18) column with acetonitrile-water gradient elution.From the recovery achieved,the best solid phase cartridge was found.Result: This method consists of using 40% methanol as the wash solvent,and 80% methanol for the elution.Among the three kinds of solid-phase being tested,Waters Oasis HLB cartridge was found to be the best one.Conclusion: The average extraction recovery of the Waters Oasis HLB cartridges was between 103%-113%,it can be used in the analysis of ginsenoside Re in plasma samples.

关键词(KeyWords): 固相萃取;人参皂苷Re;血浆样品;HPLC
solid-phase extraction;ginsenoside Re;plasma sample;HPLC

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作者(Author): 茅向军,章晨峰,孙棣,盛龙生,王广基,刘文英
MAO Xiang-jun,ZHANG Chen-feng,SUN Di,SHENG Long-sheng,WANG Guang-ji,LIU Wen-ying(China Pharmaceutical University,Nanjing 210009,China)

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