中国中药杂志

2005, (11) 839-841

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管花肉苁蓉中松果菊苷和毛蕊花糖苷的含量测定
The determination of echinacoside and acteoside in herbs of Cistanche tubulosa

陈敏,肖苏萍,崔光红,张思巨,吴志刚,黄璐琦,刘铭庭
CHEN Min, XIAO Su-ping, CUI Guang-hong, ZHANG Si-ju, WU Zhi-gang, HUANG Lu-qi, LIU Ming-ting (Institute of Chinese Materia Medico, Chinese Academy of Traditional Chinese Medicine, Beijing 100700, China; Xinjiang Yutian Dayun Plantation, Yutian 848000, China)

摘要(Abstract):

目的:采用高效液相色谱法,测定管花肉苁蓉野生种和栽培种以及人工种植不同寄主、不同大小药材的有效成分松果菊苷和毛蕊花糖苷含量。方法:AgilentEclipseXDB-C18色谱柱(4.6mm×250mm,5μm),流动相甲醇-乙腈-1%醋酸(15:10:75),流速0.6mL·min-1,柱温30℃,检测波长334nm。结果:松果菊苷在0.904-9.04μg呈良好线性关系,回归方程为Y=2.000×106X-114800(r=0.9999),毛蕊花糖苷在1.27-12.7μg呈良好线性关系,回归方程为Y=3.000×106X-243200(r=0.9999),加样回收率松果菊苷平均为98.9%(n=5,RSD1.9%);毛蕊花糖苷平均为97.0%(n=5,RSD0.97%)。结论:本法用于管花肉苁蓉的测定简便、快速、灵敏、重复性好,所测样品中有效成分松果菊苷比毛蕊花糖苷的含量均高,野生种的2种有效成分含量均比栽培种的高,将样品以个体长度分成大、中、小3种不同样品,所测结果表示其含量按大、中、小依次增加,不同寄主间的含量差异很大,为评价管花肉苁蓉的药材质量提供一定的依据。
Objective: To analyse the echinacoside and acteoside content of Cistanche tubulosa, collected from different hosts and different size of the cultivated, which is compared to the wilding by RP-HPLC method. Method: An Agilent Eclipse XDB-C18(4.6 mm × 250 mm,5 μm) column was used and a mixture of methanol-acetonitrile-1 % acetic acid (15-10:75) was used as the mobile phase at a flow rate of 0.6 mL·min-1. The column temperature was 30℃ and the UV detection wavelength was 334 nm. Result: The calibration curves of echinacoside and acteoside were in good linearity over the range of 0.904-9.04 μg ( r = 0.999 9) , and 1.27-12.7μg ( r = 0.999 9) respectively and the average recoveries of echinacoside (and acteoside) were 98.9%(n= 5,RSD 1.9%), and 97.0%(n = 5, RSD 0.97%). Conclusion: The mothod is simple, quick, acurate. In all of the samples, the contents of echinacoside is markedly more than that of acteoside, the content of the two active component in the wilding is higher than that in the planting. The content of sample in the different sizes gradually increase from the big to the small, and the contents of samples collected from the different hosts vary markedly. These results are useful for the quality evaluation of medicinal materials of C. tubulosa.

关键词(KeyWords): 管花肉苁蓉;高效液相色谱法;松果菊苷;毛蕊花糖苷;含量测定
Cistanche tubulosa; echinacoside; acteoside; HPLC; assay

Abstract:

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基金项目(Foundation): 科技部“十五”攻关项目(2001BA701A09a)

作者(Author): 陈敏,肖苏萍,崔光红,张思巨,吴志刚,黄璐琦,刘铭庭
CHEN Min, XIAO Su-ping, CUI Guang-hong, ZHANG Si-ju, WU Zhi-gang, HUANG Lu-qi, LIU Ming-ting (Institute of Chinese Materia Medico, Chinese Academy of Traditional Chinese Medicine, Beijing 100700, China; Xinjiang Yutian Dayun Plantation, Yutian 848000, China)

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