中国中药杂志

2007, (13) 1292-1295

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大孔吸附树脂分离纯化菝葜总黄酮
Study on separation and purification of total flavones from Smilax china by macroporous absorption resin

李文霞;颜彦;叶晓川;杨祥良;
LI Wen-xia1,YAN Yan2,YE Xiao-chuan2,YANG Xiang-liang2(1.Department of Chemistry,Wuhan 430074,China;2.Life Science and Technology College,Huazhong University of Science and Technology,Wuhan 430074,China)

摘要(Abstract):

目的:研究大孔吸附树脂富集、纯化菝葜总黄酮的工艺条件及参数。方法:以总黄酮收率为指标,对工艺条件进行了筛选。结果:6种大孔吸附树脂和1种聚酰胺中,D140型树脂具有最佳的吸附及洗脱参数,其静态吸附容量以干树脂计为14.87 mg.g-1。最佳吸附条件:药液质量浓度为0.5 g.mL-1(以生药量计),吸附流速为2 BV.h-1,吸附量为18 BV;洗脱最佳条件:以10 BV蒸馏水、5 BV的20%乙醇依次洗脱,弃去洗脱液;再用5~10BV,60%乙醇以流速1 BV.h-1进行洗脱,总黄酮的收率为84.72%。结论:D140型树脂综合性能较好,适于菝葜总黄酮的分离纯化。
Objective: To investigate the technological parameters of the purification process of total flavones from Smilax china with macroporous absorption resin.Method: The technical process for purification of total flavones with the optimum macroporous absorption resin was screened by yield of total flavones product.Result: The D140 macroporous absorption resin had the best separating efficiency when the flavones content in the liquid was 0.5 g·mL-1 equivalent to raw material,the volume of drug 18 BV(resin bad volume) with the adsorption-power 2 BV·h-1,and the volume of 60%(mL·mL-1) ethanol as eluant 5~10 BV(resin bad volume) with desorption-power 1 BV·h-1.The obtained flavones product has total flavones recovery rate of 84.72%.Conclusion: The treatment of regenerated resin is easy,this method is advisable.

关键词(KeyWords): 大孔吸附树脂;菝葜总黄酮;分离纯化
macroporous absorption resin;total flavones of Smilax china;separation and purification

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作者(Author): 李文霞;颜彦;叶晓川;杨祥良;
LI Wen-xia1,YAN Yan2,YE Xiao-chuan2,YANG Xiang-liang2(1.Department of Chemistry,Wuhan 430074,China;2.Life Science and Technology College,Huazhong University of Science and Technology,Wuhan 430074,China)

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