中国中药杂志

2010, v.35(06) 708-710

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HPLC同时测定小柴胡汤中5种有效成分
Simultaneous determination of five active constitutents in Xiaochaihu Tang by HPLC

刘青春;赵军宁;鄢良春;易进海;宋军;
LIU Qingchun2,ZHAO Junning1*,YAN Liangchun1,YI Jinhai1,SONG Jun1(1.Sichuan Academy of Chinese Medicine Sciences,Chengdu 610041,China;China;2.WestChina-Frontier Pharma Tech Co,Ltd,Chengdu 610041,China)

摘要(Abstract):

目的:建立小柴胡汤中黄芩苷、黄芩素、汉黄芩苷、汉黄芩素和甘草酸的HPLC-PDA测定方法。方法:采用反相高效液相色谱法,Symmetry ShieldTMRP18色谱柱(4.6 mm×250 mm,5.0μm);流动相乙腈-0.01%磷酸溶液梯度洗脱;流速0.45 mL.min-1;检测波长251 nm;柱温30℃。结果:本方法的准确度、精密度、重复性、专属性和定量线性范围均符合要求,同时测定了小柴胡汤中黄芩苷、黄芩素、汉黄芩苷、汉黄芩素和甘草酸的含量。结论:该方法简便快速、准确可靠,可用于同时测定小柴胡汤中5种有效成分的含量。
Objective:To establish a HPLC-PDA method for the determination of baicalin,wogonoside,baicalein,wogonin and glycyrrhizic acid in Xiaochaihu Tang.Method: A Symmetry ShieldTM RP18(4.6 mm×250 mm,5.0 μm)was used with a mobile phase of acctonitrile-0.01%H3PO4 in gradient elution.The detection wavelength was 251 nm,the flow rate was 0.45 mL·min-1 and the column temperature was maintained at 30 ℃.Result: The accuracy,precision,sensitivity,specificity and linearity of this method met the requirements.The contents of the five effective fractions were determined simultaneously.Conclusion: The method is rapid,simple and accurate and it can be suitable for the determination of baicalin,wogonoside,baicalein,wogonin and glycyrrhizic acid in Xiaochaihu Tang simultaneously.

关键词(KeyWords): 小柴胡汤;黄芩苷;黄芩素;汉黄芩苷;汉黄芩素;甘草酸;高效液相色谱法
Xiaochaihu Tang;baicalin;baicalein;wogonoside;wogonin;glycyrrhizic acid;HPLC

Abstract:

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基金项目(Foundation): 国家自然科学基金项目(30672651)

作者(Author): 刘青春;赵军宁;鄢良春;易进海;宋军;
LIU Qingchun2,ZHAO Junning1*,YAN Liangchun1,YI Jinhai1,SONG Jun1(1.Sichuan Academy of Chinese Medicine Sciences,Chengdu 610041,China;China;2.WestChina-Frontier Pharma Tech Co,Ltd,Chengdu 610041,China)

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