中国中药杂志

2011, v.36(04) 429-433

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护肝布祖热颗粒提取新工艺及其对小鼠肝损伤保护作用的研究
Study on new extraction technology and protective effect of Hugan Buzure granule on liver injury in mice

吴涛;蒋岚;阿吉艾克拜尔·艾萨;孙玉华;
WU Tao1,JIANG Lan1,AISA Hajiakber 1*,SUN Yuhua2(1.Key Laboratory of Plant Resources and Chemistry of Arid Zone,Xinjiang Technical Institute of Physics and Chemistry,Chinese Academy of Sciences,Urumqi 830011,China; 2.Xinjiang Institute of Materia Medica,Urumqi 830002,China)

摘要(Abstract):

目的:确定护肝布祖热颗粒提取新工艺,比较不同提取物对小鼠肝损伤的保护作用。方法:通过正交试验确定护肝布祖热颗粒中挥发油的提取工艺、挥发油包合条件、乙醇提取工艺。建立BCG+LPS诱导免疫肝损伤小鼠模型和CCl4致小鼠急性肝损伤模型,检测血清中ALT和AST水平,测定肝匀浆中MDA,SOD含量,取肝脏称重计算肝体指数,确定护肝布祖热颗粒提取物对肝脏损伤的保护作用。结果:挥发油提取工艺为加入12倍量水,浸泡2 h后提取8 h。挥发油的最佳包合工艺为挥发油与β-环糊精(β-CD)1∶6,包合温度40℃,包合时间1 h。乙醇提取的最佳工艺为10倍量50%的乙醇提取3次,每次2 h。与模型组相比,新工艺提取物可明显降低免疫性肝损伤小鼠血清中ALT(P<0.01)和AST(P<0.05)活性,升高肝匀浆SOD活性(P<0.05),可降低CCl4致小鼠急性肝损伤模型中AST活性(P<0.01)。结论:护肝布祖热颗粒提取新工艺可行,用该工艺制备的颗粒对小鼠肝损伤具有保护作用,药效作用优于原工艺提取物。
Objective:To study on the new preparation technology of Hugan Buzure granule and to compare protective effect on liver injury in rats by different extraction processes.Method:Volatile oil extraction technology,inclusion condition and ethanol extraction condition were selected by orthogonal experiments.The experiment models of liver injury were induced by carbon tetrachloride,bacillus calmette-guerin(BCG) and plus lipolysaccharides(LPS) in rats,respectively.ALT,AST in serum,and MDA,SOD in liver were measured and the rats were killed to calculate the liver coefficient to evaluate the protective effect of Hugan Buzure granule on experimental injury in rats.Result:The optimum conditions of volatile oil extraction were 1∶12 of solid-liquid ratio,2 h of soaking time,and 8 h of extracting time.The optimal β-cyclodextrin inclusion complex condition was as follows:the volatile oils formed complex with the β-CD in a ratio of 1:6 and stirring for 1 h at 40 ℃.The optimum ethanol extraction was as follows:refluxing and extracting 3 times with 10-fold 50% ethanol,2 h for each time.Compared with the model group,the new technology extraction of Hugan Buzure granule could obviously inhibite the elevation of serum ALT(P<0.01),AST(P<0.05) of liver injury induced by BCG+LPS,and elevate the contents of SOD,and obviously inhibite the elevation of serum AST(P<0.01) of liver injury induced by carbon tetrachloride.Conclusion:The new preparation technology was feasible.The new extraction could protect the liver injury in rats,which was better than extraction of current preparation technology.

关键词(KeyWords): 护肝布祖热颗粒;提取工艺;肝损伤
Hugan Buzure granule;extraction technology;liver injury

Abstract:

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基金项目(Foundation): 国家科技支撑计划项目(2006BAI06A17-09);; 新疆维吾尔自治区重大专项(200733146-4)

作者(Author): 吴涛;蒋岚;阿吉艾克拜尔·艾萨;孙玉华;
WU Tao1,JIANG Lan1,AISA Hajiakber 1*,SUN Yuhua2(1.Key Laboratory of Plant Resources and Chemistry of Arid Zone,Xinjiang Technical Institute of Physics and Chemistry,Chinese Academy of Sciences,Urumqi 830011,China; 2.Xinjiang Institute of Materia Medica,Urumqi 830002,China)

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