中国中药杂志

2017, v.42(01) 100-106

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超滤-纳滤联用优化益母草生物碱的浓缩工艺
Optimize concentrate process of alkaloid from Leonurus japonicus by ultrafultration-nanofiltration coupling technology

李存玉;马赟;刘奕洲;李红阳;顾佳美;彭国平;
LI Cun-yu;MA Yun;LIU Yi-zhou;LI Hong-yang;GU Jia-mei;PENG Guo-ping;College of Pharmacy,Nanjing University of Chinese Medicine;Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization;

摘要(Abstract):

采用超滤-纳滤联用技术浓缩益母草中生物碱,通过响应面法建立二次回归模型,优化浓缩工艺。实验表明通过超滤预处理,益母草水提液中总蛋白去除率94.38%,纳滤技术相较于传统热浓缩优势明显,益母草生物碱的最佳浓缩工艺为截留相对分子质量450,p H 3.07,盐酸水苏碱质量浓度80.15 mg·L-1,总生物碱质量浓度285.73 mg·L-1,在此条件下,盐酸水苏碱和总生物碱的截留率分别为93.37%,95.85%,相对误差为0.79%,1.16%,Box-Behnken试验设计方法可以用于益母草生物碱的浓缩工艺优化,纳滤浓缩为热敏性中药成分的分离精制提供技术支撑。
To optimize the concentrate process of alkaloid from Leonurus japonicus by nanofiltration-ultrafiltration coupling technology with response surface methodology. The experiment showed that after ultrafiltration pre-treatment,the total protein removal rate was94. 38% in aqueous extract from L. japonicus,and the nanofiltration technology had obvious advantages over the conventional concentrate process. The optimal concentrate conditions were as follows:molecular weight cut-off 450,p H 3. 07,concentration of stachydrine hydrochloride 80. 15 mg·L-1,and concentration of the total alkaloid 285. 73 mg·L-1. The cut-off rate was 93. 37% and 95. 85% respectively for stachydrine hydrochloride and the total alkaloid under the optimum conditions,with a relative error of 0. 79% and 1. 16%respectively. The combination of Box-Behnken design and response surface analysis can well optimize the concentrate process of L. japonicus by nanofiltration,and the results provide the basis for nanofiltration concentrate for heat-sensitive traditional Chinese medicine.

关键词(KeyWords): 生物碱;益母草;盐酸水苏碱;纳滤;浓缩;超滤
alkaloid;Leonurus japonicas;stachydrine hydrochloride;nanofiltration;concentrate;ultrafiltration

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(81503258);; 江苏省自然科学基金青年基金项目(BK20151005);; 江苏省中医药局科技项目(YB2015009)

作者(Author): 李存玉;马赟;刘奕洲;李红阳;顾佳美;彭国平;
LI Cun-yu;MA Yun;LIU Yi-zhou;LI Hong-yang;GU Jia-mei;PENG Guo-ping;College of Pharmacy,Nanjing University of Chinese Medicine;Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization;

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DOI: 10.19540/j.cnki.cjcmm.20161222.057

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