中国中药杂志

2017, v.42(19) 3718-3722

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密银花对照药材标定标准的研究
Study on calibration standard for reference drug of Lonicera japonica cultivated in Xinmi

杨健;蒋超;金艳;赵玉洋;袁媛;黄璐琦;
YANG Jian;JIANG Chao;JIN Yan;ZHAO Yu-yang;YUAN Yuan;HUANG Lu-qi;State Key Laboratory Breeding Base of Dao-di Herbs,National Resource Center for Chinese Materia Medica,China Academy of Chinese Medical Sciences;

摘要(Abstract):

建立道地药材密银花对照药材标定技术规范,作为密银花对照药材内在质量控制和评价的理论依据及技术标准,为道地药材现代研究及其特征辨识提供支撑。收集10批密银花,根据2015年版《中国药典》一部方法进行检验,10批样品均符合规定;建立LC-MS指纹图谱检测方法,确定了23个共有峰,并根据对照品和参考文献指认了8个共有峰,对10批密银花药材相似度进行了考察,其相似度在0.95以上。使用7对金银花种质鉴定核心引物进行SSR指纹图谱构建,10批密银花指纹图谱均与河南尖山大毛花种质SSR参照图谱差异位点数为1或0个,符合密银花种质分子特征。通过结合DNA指纹图谱和LC-MS化学指纹图谱,建立了首个中药道地药材的标定标准,该标定标准的制定为加快中药标准药材尤其是道地药材的评价体系的建设提供了理论依据及技术标准。
The calibration technical specification for reference drug of Lonicera japonica cultivated in Xinmi( Mi Yin Hua) established in this study can be used as the theoretical basis and technical standard for internal quality control and evaluation of reference drug of Mi Yin Hua and for modern research and characteristics identification of Dao-di herbs. Based on the quality standard of L. japonica in Chinese Pharmacopoeia( version 2015),10 batches samples of Mi Yin Hua also conformed to the stipulation. The LC-MS fingerprint common mode of Mi Yin Hua was obtained,and a total of 23 characteristic peaks were selected as the common fingerprint peaks,and eight common chromatographic peaks in the fingerprint was identified based on standard substances and references. The similarities of 10 batches samples were greater than 0. 95. Seven core primers were used to construct SSR fingerprint and the ten batches samples were only 0 or 1 site disaccord with the standard diagram of Jianshan Damaohua germplasm from Xinmi. This paper constructed the first calibration standard of Dao-di herbals by combination of macroscopical identification,SSR fingerprint and LC-MS fingerprint,and the calibration standard provide theoretical basis and technical standard for evaluation system of Dao-di herbals.

关键词(KeyWords): 金银花;密银花;道地药材;指纹图谱
Lonicera japonica;Mi Yin Hua;Dao-di herbs;fingerprint

Abstract:

Keywords:

基金项目(Foundation): 科技基础性工作专项(2015FY111500);; 中央级公益性科研院所基本科研业务费专项资金项目(ZZ10-008)

作者(Author): 杨健;蒋超;金艳;赵玉洋;袁媛;黄璐琦;
YANG Jian;JIANG Chao;JIN Yan;ZHAO Yu-yang;YUAN Yuan;HUANG Lu-qi;State Key Laboratory Breeding Base of Dao-di Herbs,National Resource Center for Chinese Materia Medica,China Academy of Chinese Medical Sciences;

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