中国中药杂志

2021, v.46(20) 5291-5303

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枳壳不同提取部位对正常小鼠与胃肠动力障碍大鼠的燥性比较及谱燥关系研究
Dryness comparison of different fractions of Aurantii Fructus extract on normal mice and gastrointestinal motility disorder rats and spectrum-dryness study

乔日发;钟民勇;张敏;杨林;杜习勤;庹福临;袁金斌;
QIAO Ri-fa;ZHONG Min-yong;ZHANG Min;YANG Lin;DU Xi-qin;TUO Fu-lin;YUAN Jin-bin;Key Laboratory of Modern Preparation of Traditional Chinese Medicine Under Ministry of Education, Jiangxi University of Traditional Chinese Medicine;Nanchang Key Laboratory of Quality Control and Safety Evaluation of Traditional Chinese Medicine, Nanchang Institute for Food and Drug Control;College of Pharmacy, Jiangxi University of Traditional Chinese Medicine;

摘要(Abstract):

枳壳为常用理气中药。传统中医药理论与现代实验研究均表明枳壳具有燥性作用,但其燥性物质基础尚不明确。近年来,谱效关系研究已经被广泛运用于中药药效物质研究,其具体研究思路与方法也在不断完善。基于谱效学研究思路,该文以枳壳不同提取部位为研究对象,首先建立其超高效液相色谱-四极杆-飞行时间质谱(UHPLC-Q-TOF-MS)指纹图谱并进行全成分分析,接着系统比较其对正常小鼠和胃肠动力障碍(GMD)大鼠的燥性作用,最后整合主成分分析(PCA)、皮尔逊双变量相关性分析和正交偏最小二乘分析(OPLS)等3种化学计量学手段,关联谱-燥,基本明确枳壳燥性成分。结果表明芸香柚皮苷、柚皮苷、柚皮素、枸橘苷、氧化前胡素和圣草酚-7-O-葡糖苷对肾脏水通道蛋白2(AQP2)、结肠水通道蛋白3(AQP3)和颌下腺水通道蛋白5(AQP5)的表达有较显著的相关性和贡献度,为枳壳中主要的燥性成分。
Aurantii Fructus is a commonly used qi-regulating medicinal herb in China. Both traditional Chinese medicine theory and modern experimental research demonstrate that Aurantii Fructus has dryness effect, the material basis of which remains unclear. In recent years, spectrum-effect relationship has been widely employed in the study of active ingredients in Chinese medicinal herbs, the research ideas and methods of which have been constantly improved. Based on the idea of spectrum-effect study, the ultra-high perfor-mance liquid chromatography-quadrupole-time of flight mass spectrometry(UHPLC-Q-TOF-MS) fingerprints of different fractions of Aurantii Fructus extract were established for the identification of total components. Then, the dryness effects of the fractions on normal mice and gastrointestinal motility disorder(GMD) rats were systematically compared. Finally, principal component analysis(PCA), Pearson bivariate correlation analysis and orthogonal partial least squares analysis(OPLS) were integrated to identify the dryness components of Aurantii Fructusextract. The results showed that narirutin, naringin, naringenin, poncirin, oxypeucedanin, and eriodictyol-7-O-glucoside had significant correlations with and contributed to the expression of AQP2 in kidney, AQP3 in colon, and AQP5 in submandibular gland, which were the main dryness components in Aurantii Fructus.

关键词(KeyWords): 枳壳;燥性;谱-燥关系;物质基础;水通道蛋白;胃肠动力障碍
Aurantii Fructus;dryness effect;spectrum-dryness relationship;material basis;aquaporin;gastrointestinal motility disorder

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基金项目(Foundation): 国家自然科学基金项目(81860698)

作者(Author): 乔日发;钟民勇;张敏;杨林;杜习勤;庹福临;袁金斌;
QIAO Ri-fa;ZHONG Min-yong;ZHANG Min;YANG Lin;DU Xi-qin;TUO Fu-lin;YUAN Jin-bin;Key Laboratory of Modern Preparation of Traditional Chinese Medicine Under Ministry of Education, Jiangxi University of Traditional Chinese Medicine;Nanchang Key Laboratory of Quality Control and Safety Evaluation of Traditional Chinese Medicine, Nanchang Institute for Food and Drug Control;College of Pharmacy, Jiangxi University of Traditional Chinese Medicine;

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