中国中药杂志

2015, v.40(11) 2062-2067

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金银花组蛋白甲基转移酶基因生物信息学及表达分析
Bioinformatics analysis and expressed level of histone methyltransferase genes in Lonicera japonica

齐琳洁;袁媛;黄璐琦;龙平;查良平;王尧龙;
QI Lin-jie;YUAN Yuan;HUANG Lu-qi;LONG Ping;ZHA Liang-ping;WANG Yao-long;State Key Laboratory of Dao-di Herbs Breeding Base,National Resources Center for Chinese Materia Medica,China Academy of Chinese Medical Sciences;

摘要(Abstract):

该研究从金银花转录组数据库中获得23个组蛋白甲基转移酶基因,分别对其核苷酸特性、蛋白理化性质、亚细胞定位、二级结构及保守域、系统进化和基因表达情况进行分析。其中系统进化分析结果表明,23个金银花组蛋白甲基转移酶可以分为2类:赖氨酸甲基转移酶和精氨酸甲基转移酶。基因表达分析结果表明金银花组蛋白甲基转移酶基因具有明显的种间表达偏好性和器官表达偏好性,即23个组蛋白甲基转移酶基因在金银花花蕾中表达量均高于红金银花,但在金银花叶中表达量均低于红金银花,并获得8个花蕾特异性表达基因,为进一步了解金银花类药用植物中组蛋白甲基转移酶的功能以及活性成分表观调控提供依据。
Twenty-three histone methyltransferase genes were obtained from transcriptome dataset of Lonicera japonica. The nucleotide and proteins characteristics,subcellular localization,senior structural domains and conservative forecasting were analyzed. The result of phylogenetic tree showed that 23 histone methyltransferases were mainly divided into two groups: lysine methyltransferase and arginine methyltransferases. The result of gene expression showed that 23 histone methyltransferases showed preference in terms of interspecies and organs. They were more expressed in buds of L. japonica than in L. japonica var. chinensis and lower in leaves of L. japonica than in L. japonica var. chinensis. Eight genes were specific expressed in flower. These results provided basis for further understanding the function of histone methyltransferase and epigenetic regulation of active ingredients of L. japonica.

关键词(KeyWords): 金银花;组蛋白甲基转移酶;生物信息学
Lonicera japonica;histone methyltransferase;bioinformatics

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(81373959,81325023);; 中医药行业科研专项(201407003)

作者(Author): 齐琳洁;袁媛;黄璐琦;龙平;查良平;王尧龙;
QI Lin-jie;YUAN Yuan;HUANG Lu-qi;LONG Ping;ZHA Liang-ping;WANG Yao-long;State Key Laboratory of Dao-di Herbs Breeding Base,National Resources Center for Chinese Materia Medica,China Academy of Chinese Medical Sciences;

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