范胜军;顾袁琴;李国玉;张明宇;李宝馨;张红;侯云龙;
FAN Shengjun1,GU Yuanqin1,LI Guoyu1,ZHANG Mingyu1,LI Baoxin1,ZHANG Hong2,HOU Yunlong1(1.Department of Pharmacology,Harbin Medical University,Harbin 150086,China; 2.First Affiliated Hospital of Harbin Medical University,Harbin 150001,China)

• 1:哈尔滨医科大学药学院药理教研室
• 2:哈尔滨医科大学附属第一医院

摘要(Abstract)：

目的:建立了测定大鼠血浆和房水中芒果苷含量的液相色谱检测方法。方法:以对硝基苯酚作内标,甲醇-2%冰醋酸(40∶60)为流动相,流速为1.0 mL.min-1。在Cosmosil ODS C18色谱柱(4.6 mm×250 mm,5μm)上进行分离。结果:方法的线性范围分别为血浆0.50～250.00 mg.L-1,房水0.10～10.00 mg.L-1,线性回归系数均在0.995 4以上,日内和日间精密度RSD均小于12%,最低检量限0.05 mg.L-1。结论:本方法具有简便、准确等优点,可满足芒果苷在大鼠血浆和房水中药动学研究的需求。
A HPLC method was developed for the determination of mangiferin in rat plasma and aqueous humor.4-Nitrophenol was used as internal standard.Analysis was performed on a Cosmosil ODS C18 analytical column(4.6 mm×250 mm,5 μm) with mobile phase consisting of methanol-water(40∶60) with 2% glacial acetic acid at a flow rate of 1.0 mL·min-1.The calibration curve of mangiferin in rat plasma and aqueous humor showed excellent linear behaviors over the investigated concentration of 0.50-250.00 mg·L-1 in plasma and 0.10-10.00 mg·L-1 in aqueous humor,respectively,and the correlation coefficients were all above 0.995 4.The intra-day and inter-day precisions for all samples were measured to be below 12%.The limit of quantitation was 0.10 mg·L-1 and low enough for the determination of mangiferin in all samples.The validated method has been successfully applied to preliminary pharmacokinetics study of mangiferin in rat plasma and aqueous humor.

关键词(KeyWords)： 芒果苷;药动学;高效液相色谱法;血浆;房水
mangiferin;pharmacokinetics;HPLC;plasma;aqueous humor

Abstract:

Keywords:

基金项目(Foundation):

作者(Authors): 范胜军;顾袁琴;李国玉;张明宇;李宝馨;张红;侯云龙;
FAN Shengjun1,GU Yuanqin1,LI Guoyu1,ZHANG Mingyu1,LI Baoxin1,ZHANG Hong2,HOU Yunlong1(1.Department of Pharmacology,Harbin Medical University,Harbin 150086,China; 2.First Affiliated Hospital of Harbin Medical University,Harbin 150001,China)

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