中国中药杂志

2016, v.41(15) 2895-2900

[打印本页] [关闭]
本期目录(Current Issue) | 过刊浏览(Past Issue) | 高级检索(Advanced Search)

覆盆子有效部位对去卵巢AD小鼠海马蛋白组学影响研究
Effects of extractionfrom raspberry on hippocampus proteomics of mice suffered from ovariectomized-induced AD

侯敏;傅应军;刘超;韦益飞;陈银芳;谢一辉;黄丽萍;
HOU Min;FU Ying-jun;LIU Chao;WEI Yi-fei;CHEN Yin-fang;XIE Yi-hui;HUANG Li-ping;College of Pharmacy,Jiangxi University of Chinese Medicine;

摘要(Abstract):

该实验为探究覆盆子乙酸乙酯提取物对去卵巢阿尔茨海默病(AD)模型小鼠海马蛋白组表达的影响。小鼠去卵巢后按体重随机分成假手术组、模型组、阳性药对照组(雌二醇,0.1 g·L(-1))和覆盆子乙酸乙酯部位组(生药浓度18 g·kg(-1))和覆盆子乙酸乙酯部位组(生药浓度18 g·kg(-1))。阳性对照组小鼠隔天皮下注射雌二醇0.2 m L/只,覆盆子乙酸乙酯部位组灌胃覆盆子乙酸乙酯提取物0.01 m L·g(-1))。阳性对照组小鼠隔天皮下注射雌二醇0.2 m L/只,覆盆子乙酸乙酯部位组灌胃覆盆子乙酸乙酯提取物0.01 m L·g(-1),模型组与假手术组灌胃生理盐水0.01 m L·g(-1),模型组与假手术组灌胃生理盐水0.01 m L·g(-1),每日1次,连续给药32 d。分离小鼠海马,提取蛋白,Nanol-ESI的液相-质谱联用系统检测,Protein Discovery软件进行蛋白鉴定,SIEVE软件对海马蛋白进行相对定量定性分析。实验结果显示,覆盆子乙酸乙酯组与模型组相比较共有66个差异表达蛋白,包含热休克蛋白、微管蛋白、能量代谢相关蛋白和脑保护相关蛋白等与AD相关的蛋白。以上差异蛋白可能是覆盆子有效部位防治AD的靶点蛋白。
This paper was aimed to investigate the impact of the extraction from raspberry on the Alzheimer disease model protein expression. According to weight,the ovariectomized mice were randomly divided into shame operation group,model group,estrogen positive control group(0. 1 g·L(-1),每日1次,连续给药32 d。分离小鼠海马,提取蛋白,Nanol-ESI的液相-质谱联用系统检测,Protein Discovery软件进行蛋白鉴定,SIEVE软件对海马蛋白进行相对定量定性分析。实验结果显示,覆盆子乙酸乙酯组与模型组相比较共有66个差异表达蛋白,包含热休克蛋白、微管蛋白、能量代谢相关蛋白和脑保护相关蛋白等与AD相关的蛋白。以上差异蛋白可能是覆盆子有效部位防治AD的靶点蛋白。
This paper was aimed to investigate the impact of the extraction from raspberry on the Alzheimer disease model protein expression. According to weight,the ovariectomized mice were randomly divided into shame operation group,model group,estrogen positive control group(0. 1 g·L(-1)) and ethyl acetate extraction part control group(in dose of 18 g·kg(-1)) and ethyl acetate extraction part control group(in dose of 18 g·kg(-1)). Each mouse in positive control group was subcutaneous injected of estradiol with 0. 2 m L every two days. Raspberry effective parts group were given 0. 01 m L·g(-1)). Each mouse in positive control group was subcutaneous injected of estradiol with 0. 2 m L every two days. Raspberry effective parts group were given 0. 01 m L·g(-1)raspberry ethylacetate extracts,model group and control group were given 0. 01 m L·g(-1)raspberry ethylacetate extracts,model group and control group were given 0. 01 m L·g(-1)saline once a day. The drug administration lasted for 32 days. Proteins from mice' s hippocampus were extracted,then Nanol-ESI liquid-mass spectrometry system was used for detection and Protein Discovery software was used for identification to qualitative analysis different groups of hippocampal proteins by using the software of SIEVE. The results showed that model group compared with the mice of ethyl acetate extraction part control group have 66 differentially expressed proteins including heat shock protein,microtubule protein,protein involved in energy metabolism and protein of brain protection related proteins associated with AD. Those differences protein may be the target that Raspberry prevention and treatment of AD.

关键词(KeyWords): 覆盆子;AD;海马;蛋白质组学
raspberry;AD;hippocampus;proteomics

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(30960496);; 江西省自然科学基金项目(20133BAB20002,20142BAB205025);; 江西省卫生厅中医药科研计划项目(2013A010);; 江西省高等学校大学生创新创业计划项目(201310412005)

作者(Authors): 侯敏;傅应军;刘超;韦益飞;陈银芳;谢一辉;黄丽萍;
HOU Min;FU Ying-jun;LIU Chao;WEI Yi-fei;CHEN Yin-fang;XIE Yi-hui;HUANG Li-ping;College of Pharmacy,Jiangxi University of Chinese Medicine;

参考文献(References):

扩展功能
本文信息
服务与反馈
本文关键词相关文章
本文作者相关文章
中国知网
分享