玄参提取物化学成分及其大鼠血浆移行成分的LC-LTQ-Orbitrap分析LC-LTQ-Orbitrap analysis on chemical constituents in Scrophulariae Radix extract and their metabolites in rat plasma
徐多多;庞欢欢;江美芳;简伟杰;王勤辉;孙璐;董姊怡;黄建梅;
XU Duo-duo;PANG Huan-huan;JIANG Mei-fang;JIAN Wei-jie;WANG Qin-hui;SUN Lu;DONG Zi-yi;HUANG Jian-mei;School of Chinese Materia Medica,Beijing University of Chinese Medicine;
摘要(Abstract):
运用HPLC-LTQ-Orbitrap技术,分析玄参提取物体外化学成分及给药后大鼠血中移行成分。采用Venusil MP C18色谱柱梯度洗脱进行分离,负离子模式下采集谱图。通过与文献及部分对照品比对,综合分析总离子流色谱图、提取离子流图以及质谱图等信息,对玄参提取物的化学成分及其血中移行成分进行定性研究。共推断出玄参提取物中37个化学成分,其中12个环烯醚萜苷类成分,20个苯丙素类成分,5个未知成分;确定哈巴苷、哈巴俄苷和安格洛苷C均以原型入血,并推测出其他2个原型成分及2个代谢产物。该研究能够为阐明玄参的药效物质基础提供一定依据,也对进一步研究玄参有重要意义。
Chemical constituents in extract of Scrophulariae Radix and their metabolites in rat plasma after oral administration were identified by HPLC-LTQ-Orbitrap. Samples were separated by a Venusil MP C18 column using a binary gradient elution. The information on the total ion chromatogram,the extraction chromatogram and the mass spectrogram in a negative mode were synthetically analyzed by comparing the retention time,MS and MS / MS spectra with literature data and some of reference standards to conduct a qualitative study on constituents of Radix Scrophulariae extract in vivo and in vitro. Totally 37 compounds from Scrophularia ningpoensis extract were detected including 12 iridoid glycosides,20 phenylpropanoids and 5 unknown compounds. In vivo,harpagide,harpagoside and angoroside C were confirmed to enter into the blood in prototype forms. Besides,another 2 prototype compounds and 2 metabolites were detected in rat plasma after oral administration of S. ningpoensis extract. The results are beneficial for the determination of bioactive substances of S. ningpoensis and significant for further studies on S. ningpoensis.
关键词(KeyWords):
玄参;LC-LTQ-Orbitrap;化学成分;入血成分
Scrophularia ningpoensis;LC-LTQ-Orbitrap;chemical component;constituents in plasma
基金项目(Foundation): 国家“重大新药创制”科技重大专项(2011ZX09201-201-22)
作者(Authors):
徐多多;庞欢欢;江美芳;简伟杰;王勤辉;孙璐;董姊怡;黄建梅;
XU Duo-duo;PANG Huan-huan;JIANG Mei-fang;JIAN Wei-jie;WANG Qin-hui;SUN Lu;DONG Zi-yi;HUANG Jian-mei;School of Chinese Materia Medica,Beijing University of Chinese Medicine;
参考文献(References):
- [1]张刘强,李医明.近10年玄参属植物化学成分和药理作用研究进展[J].中草药,2011,42(11):2360.
- [2]龚友兰,余三红,易钊旭,等.2002—2012年玄参药学研究文献计量分析[J].中国中医药图书情报杂志,2014(1):21.
- [3]龚友兰,潘琦虹,向大雄.玄参属植物中环烯醚萜类成分及其生物活性研究进展[J].天然产物研究与开发,2012,24(3):406.
- [4]陈海生,李医明,蒋山好,等.玄参中微量单萜和二萜成分[J].解放军药学学报,2000,16(1):22.
- [5]周雯,蒋林,王晓娟,等.西南产玄参药材的HPLC指纹图谱研究[J].中药材,2012,35(8):1230.
- [6]Jing J,Chan C,Xu L,et al.Development of an in-line HPLC fingerprint ion-trap mass spectrometric method for identification and quality control of Radix Scrophulariae[J].J Pharm Biomed Anal,2011,56(4):830.
- [7]Cao G,Cong X,Cai H,et al.Simultaneous quantitation of eight active components in crude and processed Radix Scrophulariae extracts by high performance liquid chromatography with diode array detector[J].Chin J Nat Med,2012,10(3):213.
- [8]张雪梅,王瑞,安睿,等.HPLC同时测定玄参中5种成分的含量[J].中国中药杂志,2011,36(6):709.
- [9]Wu Q,Yuan Q,Liu E,et al.Fragmentation study of iridoid glycosides and phenylpropanoid glycosides in Radix Scrophulariae by rapid resolution liquid chromatography with diode-array detection and electrospray ionization time-of-flight mass spectrometry[J].Biomed Chromatogr,2010,24(8):808.
- [10]张磊,李兴平,雷玲,等.丹参、黄芪、玄参对内毒素缓释致热大鼠TLR4/NF-κB信号通路活化的影响[J].中药药理与临床,2014,30(1):73.
- [11]顾伟梁,陈长勋,王樱,等.玄参水提物对心室重构大鼠心肌纤维化的影响[J].中草药,2008,39(9):1371.
- [12]Gu W L,Chen C X,Huang X Y,et al.The effect of angoroside C on pressure overload-induced ventricular remodeling in rats[J].Phytomedicine,2015,22(7/8):705.
- [13]季金玉,张云,丛晓东,等.玄参的化学成分及质量控制研究进展[J].中华中医药学刊,2010,28(12):2507.
- [14]杨宪,杨水平,张雪,等.HPLC-UV-ELSD同时测定玄参中5种成分的含量[J].中国中药杂志,2009,34(1):68.
- [15]龚友兰,向大雄,邓长凤,等.HPLC-UV双波长法同时测定玄参中5种主要成分的含量[J].中南药学,2008,6(6):660.
- [16]韩乐,许虎,刘训红,等.高效毛细管电泳测定玄参中6种指标成分的含量[J].中国药学杂志,2012,47(7):555.
- [17]宋晓晨,陈林霖,戚进,等.哈巴俄苷及其代谢产物在大鼠体内的药代动力学[J].中国药科大学学报,2011,42(3):262.
- [18]Wang S J,Ruan J X,Zhao Y H,et al.Simultaneous determination of harpagoside and cinnamic acid in rat plasma by liquid chromatography electrospray ionization mass spectrometry and its application to pharmacokinetic studies[J].Biomed Chromatogr,2008,22(1):50.
- [19]Jing J,Chan C O,Xu L,et al.Development of an in-line HPLC fingerprint ion-trap mass spectrometric method for identification and quality control of Radix Scrophulariae[J].J Pharm Biomed Anal,2011,56(4):830.
- [20]Chen Y H,Qi J,Hua J,et al.Structural characterization and identification of major constituents in Radix Scrophulariae by HPLC coupled with electrospray ionization quadrupole time-offlight tandem mass spectrometry[J].Chin J Nat Med,2014,12(1):47.
- [21]崔忠生,邸科前,马焕云.哈巴苷及哈巴俄苷对肾上腺素损伤血管内皮细胞的保护作用[J].山东医药,2009,49(25):60.
- [22]崔忠生,邸科前,马焕云.哈巴苷及哈巴俄苷对过氧化氢损伤血管内皮细胞的保护作用[J].医学研究与教育,2009,26(2):11.
- [23]李医明,曾华武,贺祥,等.玄参中环烯醚萜甙和苯丙素甙对LTB4产生及血小板聚集的影响[J].第二军医大学学报,1999,20(5):301.
- [24]王喜军.中药血清药物化学的研究动态及发展趋势[J].中国中药杂志,2006,31(10):789.
- [25]王喜军.中药及中药复方的血清药物化学研究[J].世界科学技术——中药现代化,2002,4(2):1.
- [26]钟杰,谭朝丹,王天明,等.大鼠体内生地黄吸收成分分析及其药动学研究[J].药学学报,2013(9):1464.
- [27]Zhang L,Ma Y,Liu Y,et al.Development and validation of high liquid performance chromatography-tandem mass spectrometry method for simultaneous determination of geniposidic acid and aucubin in rat plasma for pharmacokinetic study after oral administration of Du-zhong tea extract[J].J Chromatogr B,2014,963:62.
- [28]陆林玲,束艳,钱大玮,等.大鼠灌胃四逆散提取物后血浆、尿液、粪便、胆汁中主要代谢产物的鉴定[J].药学学报,2011,46(11):1374.
- [29]康桢,吴卫华,王俊杰,等.桃叶珊瑚苷及其苷元的药理研究进展[J].中国中药杂志,2007,32(24):2585.
- [30]刘军海,裘爱泳.杜仲叶中桃叶珊瑚苷提取工艺研究[J].中成药,2007,29(2):266.
- 玄参
- LC-LTQ-Orbitrap
- 化学成分
- 入血成分
Scrophularia ningpoensis - LC-LTQ-Orbitrap
- chemical component
- constituents in plasma
- 徐多多
- 庞欢欢
- 江美芳
- 简伟杰
- 王勤辉
- 孙璐
- 董姊怡
- 黄建梅
XU Duo-duo- PANG Huan-huan
- JIANG Mei-fang
- JIAN Wei-jie
- WANG Qin-hui
- SUN Lu
- DONG Zi-yi
- HUANG Jian-mei
- School of Chinese Materia Medica
- Beijing University of Chinese Medicine
- 徐多多
- 庞欢欢
- 江美芳
- 简伟杰
- 王勤辉
- 孙璐
- 董姊怡
- 黄建梅
XU Duo-duo- PANG Huan-huan
- JIANG Mei-fang
- JIAN Wei-jie
- WANG Qin-hui
- SUN Lu
- DONG Zi-yi
- HUANG Jian-mei
- School of Chinese Materia Medica
- Beijing University of Chinese Medicine