中国中药杂志

2018, v.43(17) 3525-3529

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竹节参总皂苷通过调节miR-199-5p改善小鼠脂肪性肝病的实验研究
Protective effects of total saponins of Panax japonicas on non-alcoholic fatty liver disease through regulating expression of miR-199-5p

熊海容;李聪;何春喜;何毓敏;袁成福;王婷;张长城;袁丁;刘朝奇;
XIONG Hai-rong;LI Cong;HE Chun-xi;HE Yu-min;YUAN Cheng-fu;WANG Ting;ZHANG Chang-cheng;YUAN Ding;LIU Chao-qi;Hubei Key Laboratory of Tumor Microenvironment and Immunotheraphy,Three Gorges University;Medical College,Three Gorges University;Renhe Hospital,Three Gorges University;

摘要(Abstract):

研究竹节参总皂苷(total saponins of Panax japonicas,TPSJ)对高脂高糖诱导小鼠脂肪性肝病的作用,并探究其可能的调控机制。将40只昆明小鼠(雄性)随机分为正常组,模型组,TPSJ低剂量组,TPSJ高剂量组。采用高脂高糖饲料模拟脂肪肝模型,同时采用竹节参药物低剂量100 mg·kg-1,高剂量200 mg·kg-1进行干预,喂养4周后处理动物收集血清和肝脏并检测相关生化指标。HE染色观察小鼠肝脏组织病理学变化;RT-PCR与RT-q PCR法检测肝组织中miR-199-5p,VEGFa,HGF,c-Met的转录水平;激光共聚焦显微镜法检测c-Met蛋白的表达情况。结果显示,模型组的小鼠血清ALT,TC的水平明显高于正常组,HE染色发现模型组肝组织表现出脂肪样变,脂质空泡较多,脂质明显沉积,以上现象提示脂肪肝模型成立。经过TPSJ不同剂量的干预后肝脏组织病理学变化明显改善,脂质沉积减少,肝细胞排列较为紧密;同时TPSJ能明显降低miR-199-5p的表达以及促进c-Met,HGF的表达。TPSJ可能通过调节miR-199-5p/HGF-c-Met的信号通路发挥对小鼠脂肪肝的干预作用。
To research the effection and probable mechanism for the total saponins of Panax japonicas( TPSJ) in mice on non-alcoholic fatty liver disease. Forty SPF male Kunming mice were randomily divided into four group: control group,NAFLD group,low-dose TPSJ treated group,high-dose TPSJ treated group. High-fatty and high-frutose-diet was applied to eatablish NAFLD model,and TPSJ( 100 and 200 mg·kg-1) in feeding were given for the TPSJ groups for 4 weeks. To collect the serum with liver and the ALT and TC of serum were monitored after 4 weeks. The hepatic histopathologic structure was observed by haematoxylin-eosin( HE) staining,RTPCR and RT-q PCR was applied for the detection of miR-199-5 p,VEGFa,HGF,c-Met and protein expression level was detected bv laser confocal microscope. Compared with control group,the level of serum ALT and TC in the model group was higher,the liver of the model group showed that hepatocytes display obvious lipid deposition. Then TPSJ treated showed that markedly improved histopathologic changes,decreased fatty deposition. In the meantime,the expression level of miR-199-5 p was significantly decreased,thus the expression of HGF and c-Met were significantly increased. TPSJ play a role of prevention on fatty liver,the machanism maybe by blocking miR-199-5 p targeted to c-Met signaling pathways in NAFLD.

关键词(KeyWords): 竹节参总皂苷;非酒精性脂肪性肝病;miR-199-5p;HGF;c-Met
total saponins of Panax japonicas;nonalcoholic fatty liver disease;miR-199-5p;HGF;c-Met

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(81673675);; 肿瘤微环境与免疫治疗湖北省重点实验室(三峡大学)开放基金课题(2015KZL13,2015KZL05,2015KZL06)

作者(Author): 熊海容;李聪;何春喜;何毓敏;袁成福;王婷;张长城;袁丁;刘朝奇;
XIONG Hai-rong;LI Cong;HE Chun-xi;HE Yu-min;YUAN Cheng-fu;WANG Ting;ZHANG Chang-cheng;YUAN Ding;LIU Chao-qi;Hubei Key Laboratory of Tumor Microenvironment and Immunotheraphy,Three Gorges University;Medical College,Three Gorges University;Renhe Hospital,Three Gorges University;

Email:

DOI: 10.19540/j.cnki.cjcmm.20180606.001

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