李子唯;杨野;崔秀明;廖沛然;葛进;王承潇;杨晓艳;刘大会;
LI Zi-wei;YANG Ye;CUI Xiu-ming;LIAO Pei-ran;GE Jin;WANG Cheng-xiao;YANG Xiao-yan;LIU Da-hui;Kunming Key Laboratory of Sustainable Development and Utilization of Famous-region Drug,Faculty of Life Science and Technology,Kunming University of Science and Technology;Institute of Medicinal Plants,Yunnan Academy of Agriculture Science;

• 1:昆明理工大学生命科学与技术学院昆明市道地药材可持续发展利用重点实验室
• 2:云南省农业科学院药用植物研究所

摘要(Abstract)：

以二年生三七为试验材料,水培条件下研究了镉胁迫(0,2.5,5,10μmol·L-1)对三七抗氧化酶系统和光合参数的影响及三七对镉的吸收分配规律。结果表明:2.5μmol·L-1镉处理可诱导SOD,POD,APX等酶活性的升高,10μmol·L-1镉处理则对SOD,POD,APX,CAT均产生抑制作用;镉处理对三七各部位CAT活性均具有抑制作用;三七净光合速率,蒸腾速率,气孔导度均随镉处理浓度的升高而降低,胞间CO2浓度则呈先下降后上升的趋势;三七各器官镉含量表现为须根>剪口>主根>叶片>茎;叶,茎,剪口,须根,主根镉富集系数均随镉处理浓度的升高而降低;各器官中镉含量与迁移系数均随镉处理浓度的升高而上升。综上所述,镉胁迫可对三七抗氧化酶系统和光合系统产生影响,三七对镉具有富集作用,应合理选择三七种植基地以减少对镉的吸收,合理选取三七入药部位,以降低镉摄入风险。
The physiological response and bioaccumulation of 2-year-old Panax notoginseng to cadmium stress was investigated under a hydroponic experiment with different cadmium concentrations( 0,2. 5,5,10 μmol·L-1). Result showed that low concentration( 2. 5 μmol·L-1) of cadmium could stimulate the activities of SOD,POD,APX in P. notoginseng,while high concentration( 10μmol·L-1) treatment made activities of antioxidant enzyme descended obviously. But,no matter how high the concentration of cadmium was,the activities of CAT were inhibited. The Pn,Tr,Gsin P. notoginseng decreased gradually with the increase of cadmium concentration,however Ci showed a trend from rise to decline. The enrichment coefficients of different parts in P. notoginseng ranked in the order of hair root > root > rhizome > leaf > stem,and all enrichment coefficients decreased with the increase of concentration of cadmium treatments; while the cadmium content in different parts of P. notoginseng and the transport coefficients rose. To sum up,cadmium could affect antioxidant enzyme system and photosynthetic system of P. notoginseng; P. notoginseng had the ability of cadmium enrichment,so we should plant it in suitable place reduce for reducing the absorption of cadmium; and choose medicinal parts properly to lessen cadmium intake.

关键词(KeyWords)： 三七;镉;抗氧化酶;光合参数;富集系数;迁移系数
Panax notoginseng;cadmium stress;physiological response;photosynthetic parameters;enrichment coefficients;transport coefficients

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(81360617,81460580);; 昆明理工大学分析测试基金项目(20140743)

作者(Authors): 李子唯;杨野;崔秀明;廖沛然;葛进;王承潇;杨晓艳;刘大会;
LI Zi-wei;YANG Ye;CUI Xiu-ming;LIAO Pei-ran;GE Jin;WANG Cheng-xiao;YANG Xiao-yan;LIU Da-hui;Kunming Key Laboratory of Sustainable Development and Utilization of Famous-region Drug,Faculty of Life Science and Technology,Kunming University of Science and Technology;Institute of Medicinal Plants,Yunnan Academy of Agriculture Science;

参考文献(References)：

• [1]刘刚,刘育辰,鲍建才,等.三七药理作用的研究进展[J].人参研究,2005(3):12.
• [2]袁珊珊,肖细元,郭朝晖.中国镉矿的区域分布及土壤镉污染风险分析[J].环境污染与防治,2012,34(6):51.
• [3]赵静,刘勇,张艾华,等.不同产地三七中重金属元素的含量测定及分析[J].中国中药杂志,2014,39(20):4001.
• [4]韩小丽,张小波,郭兰萍,等.中药材重金属污染现状的统计分析[J].中国中药杂志,2008,33(18):2041.
• [5]朱美霖,姜阳,崔斌,等.云南道地产区中药三七中镉的健康风险评价[C].南京:风险分析和危机反应的创新理论和方法——中国灾害防御协会风险分析专业委员会第五届年会,2012.
• [6]陈璐,米艳华,林昕,等.土壤-三七系统重金属污染调查及相关分析[J].中国中药杂志,2014,39(14):2608.
• [7]金航,崔秀明,徐珞珊,等.三七道地与非道地产区药材及土壤微量元素分析[J].云南大学学报:自然科学版,2006,28(2):144.
• [8]崔玉静,赵中秋,刘文菊,等.镉在土壤一植物一人体系统中迁移积累及其影响因子[J].生态学报,2003,23(10):2133.
• [9]熊愈辉,杨肖娥.镉对植物毒害与植物耐镉机理研究进展[J].安徽农业科学,2006,34(13):2369.
• [10]杜秀敏,殷文璇,越彦修,等.植物中活性氧的产生及清除机制[J].生物工程学报,2001,17(2):121.
• [11]王国成,曹娴.镉胁迫下植物的应答和调控[J].内蒙古环境科学,2007,19(2):40.
• [12]田国忠,李怀方,裘维蕃.植物过氧化物酶研究进展[J].武汉植物学研究,2001,19(4):332.
• [13]朱美霖,魏富刚,崔斌,等.土壤Cd胁迫对三七生长和根系DNA损伤及抗氧化酶活性的影响[J].植物资源与环境学报,2014,23(1):58.
• [14]朱美霖,陈中坚,姜阳,等.外源土壤Cd胁迫对三七富集及其药效成分的影响[J].中成药,2014,36(2):342.
• [15]H Clijsters,F V Assche.Inhibition of photosynthesis by heavy metals[J].Photosynth Res,1985,7:31.
• [16]刘俊祥,孙振元,勾萍,等.镉胁迫下多年生黑麦草的光合生理响应[J].草业学报,2012,21(3):191.
• [17]冯世静,杨途熙,张艳军,等.镉胁迫对杨树光合特性的影响[J].农业环境科学学报,2013,32(3):539.
• [18]邓培雁,刘威,韩博平.宝山堇菜(Viola baoshanensis)镉胁迫下的光合作用[J].生态学报,2007,27(5):1858.
• [19]贾雄,王国良,宗良纲,等.镉在日本无刺楤木叶片的积累与对其抗氧化系统的影响[J].四川环境,2010,29(3):13.
• [20]孙群,胡景江.植物生理学研究技术[M].杨凌:西北农林科技大学出版社,2006.
• [21]中国药典.一部[S].2010:48.
• [22]孙晓灿,魏虹,谢小红,等.水培条件下秋华柳对重金属Cd的富集特性及光合响应[J].环境科学研究,2012,25(2):220.
• [23]荆红梅,郑海雷,赵中秋,等.植物对镉胁迫响应的研究进展[J].生态学报,2001,21(12):2125.