中国中药杂志

2019, v.44(12) 2538-2543

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牦牛胆中胆汁酸类化学成分分析
Chemical profiling for bile acid derivatives in yak bile

曹妍;宋青青;李军;邓双炳;齐海军;屠鹏飞;宋月林;
CAO Yan;SONG Qing-qing;LI Jun;DENG Shuang-bing;QI Hai-jun;TU Peng-fei;SONG Yue-lin;Modern Research Center for Traditional Chinese Medicine,School of Chinese Meteria Medica,Beijing University of Chinese Medicine;State Key Laboratory of Innovative Natural Medicine and Traditional Chinese Medicine Injections,Jiangxi Qingfeng Pharmaceutical Co.,Ltd.;

摘要(Abstract):

胆汁酸为胆类中药的主要活性成分,也是多种疾病,特别是肝脏类疾病的潜在生物标志物。建立这类成分的系统分析方法,对于胆类中药药效物质的深入阐明和相关疾病的诊断及治疗均具有重要意义。该文在前期研究的基础上,着重关注于同分异构体的区分,完善胆酸类成分分析方法,并将其应用于牦牛胆中胆酸类化学成分的快速、系统表征。对于胆酸类成分的同分异构体,利用高效液相色谱串联三重四极杆-线性离子阱质谱(HPLC-Qtrap-MS)构建相对响应值-碰撞能曲线(RRCEC),发现最佳碰撞能(OCE)和半数响应碰撞能(CE_(50))均表现出较强的结构特异性,能有效地区分游离胆酸、甘氨胆酸、牛磺胆酸的同分异构体。在牦牛胆中,通过多级质谱数据分析,OCE/CE_(50)比较,保留时间匹配,并结合对照品及数据库检索,共发现并鉴定了30个胆酸类化学成分。该文建立的方法可以用于胆类中药和生物样品中胆酸类成分的快速、系统表征。
Bile acids( BAs),the major constituents of bile,are also known to be potential biomarkers of various diseases,especially liver disease. The systematic analysis of BAs is believed to be of great importance towards the clarification of the effective material basis for bile-type medicines,and the diagnosis and therapy of related diseases as well. As a part of systematic study on bile-type medicine ongoing in our group,this study lays emphasis on the isomer discrimination,and the improvement of analytical method of BAs. Further,this method was subsequently applied to elucidate in depth the chemical profile of BAs in yak bile. Regarding isomer discrimination for BAs,we constructed relative response-collision energy curves( RRCECs) by high performance liquid chromatographyion trap-time of flight-mass spectrometry( HPLC-IT-TOF-MS) in combination with high performance liquid chromatography-triple quadrupole-linear ion trap mass spectrometry( HPLC-Qtrap-MS). As a result,both the optimum collision energy( OCE) and CE_(50) exhibited great correlations with structural characteristics,thus enabling the isomer distinguishing,such as unconjugated BAs,glycine-conjugated BAs,and taurine-conjugated BAs. According to information provided by mass spectrometry,the comparison of OCE and CE_(50),retention time matching,combined with reference substances and database retrieval,a total of 30 bile acid derivatives were observed and identified in yak bile. The newly developed method could serve as a feasible tool for the in-depth characterization of BAs in bile and biological samples.

关键词(KeyWords): 胆汁酸类成分;同分异构体;牦牛胆;相对响应值-碰撞能曲线
bile acids;isomers;yak bile;relative response-collision energy curve

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(81773832);; 创新天然药物与中药注射剂国家重点实验室开放课题(QFSKL2018002);; 中国科协青年人才托举工程(2017QNRC001)

作者(Author): 曹妍;宋青青;李军;邓双炳;齐海军;屠鹏飞;宋月林;
CAO Yan;SONG Qing-qing;LI Jun;DENG Shuang-bing;QI Hai-jun;TU Peng-fei;SONG Yue-lin;Modern Research Center for Traditional Chinese Medicine,School of Chinese Meteria Medica,Beijing University of Chinese Medicine;State Key Laboratory of Innovative Natural Medicine and Traditional Chinese Medicine Injections,Jiangxi Qingfeng Pharmaceutical Co.,Ltd.;

Email:

DOI: 10.19540/j.cnki.cjcmm.20190321.202

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