中国中药杂志

2011, v.36(14) 1979-1981

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HPLC同时测定鸦胆子中3个苦木内酯的含量
Quantitative determination of contents of three components in Brucea javanica by HPLC

周中流;石任兵;刘斌;邹节明;王力生;夏敬民;
ZHOU Zhongliu1,2,SHI Renbing2,LIU Bin2,ZOU Jieming3,WANG Lisheng3,XIA Jingmin(1.Chemistry Science and Technology School,Zhanjiang Normal University,Zhanjiang 524048,China;2.School of Chinese Pharmacy Beijing University of Chinese Medicine,Beijing 100102,China;3.Guilin Sanjin Pharmaceutical Co.,Ltd.,Guilin 541004,China)

摘要(Abstract):

目的:建立鸦胆子中3种苦木内酯的高效液相色谱含量测定方法。方法:采用phenomenex C18色谱柱(4.6 mm×150 mm,5μm),甲醇-水梯度洗脱,流速1.0 mL.min-1,检测波长270 nm。结果:鸦胆苦素D在2.52~12.60μg(r=0.9996),鸦胆苦醇在2.19~10.95μg(r=0.999 6),鸦胆因H在2.91~14.55μg(r=0.999 8)线性关系良好,鸦胆因D平均回收率100.01%,RSD 0.31%(n=6);鸦胆苦醇平均回收率100.95%,RSD 1.7%(n=6);鸦胆因H平均回收率100.43%,RSD1.7%(n=6)。结论:3批样品测定结果表明,该方法简便、准确,可用于鸦胆子中3种苦木内酯的含量测定。
Objective:To develop an HPLC method for quantitative determination of three quassinoids in Brucea javanic.Method:The determination was carried out on a phenomenex C18 column(4.6 mm×250 mm,5 μm) with gradient elution program of methol-water at a flow rate of 1.0 mL·min-1,and the detection wavelength was 270 nm.Result:Linearites of bruceine D,brusatol and bruceine H were good(r=0.999 6,0.999 6,and 0.999 8) in ranges of 2.52-12.60,2.19-10.95,and 2.91-14.55 μg,respectively.The average recoveries of bruceine D,brusatol and bruceine H were 100.01%,100.95% and 100.43% respectively,and RSD of the above three compounds were 0.31%(n=6),1.7%(n=6) and 1.7%(n=6),respectively.Conclusion:The determination results of three batch samples showed that the method was simple,accurate and could be used in the quantitative determination of three components in the B.javanica.

关键词(KeyWords): 鸦胆子;苦木内酯;高效液相色谱;含量测定
Brucea javanica;quassinoid;HPLC;quantitative determination

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基金项目(Foundation): 湛江师范学院自然科学研究项目博士专项(ZL1009)

作者(Author): 周中流;石任兵;刘斌;邹节明;王力生;夏敬民;
ZHOU Zhongliu1,2,SHI Renbing2,LIU Bin2,ZOU Jieming3,WANG Lisheng3,XIA Jingmin(1.Chemistry Science and Technology School,Zhanjiang Normal University,Zhanjiang 524048,China;2.School of Chinese Pharmacy Beijing University of Chinese Medicine,Beijing 100102,China;3.Guilin Sanjin Pharmaceutical Co.,Ltd.,Guilin 541004,China)

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