中国中药杂志

2016, v.41(15) 2843-2854

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“一测多评”模式在淫羊藿药材、饮片及含淫羊藿中成药黄酮类成分检测中的一体化研究
Integrative research of QAMS model used in assay of flavonoids in Epimedii Folium,its processed products and finished Chinese medicines

冯伟红;李春;张锴镔;杨立新;易红;陈两绵;张永欣;荣立新;王智民;
FENG Wei-hong;LI Chun;ZHANG Kai-bin;YANG Li-xin;YI Hong;CHEN Liang-mian;ZHANG Yong-xin;RONG Li-xin;WANG Zhi-min;Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences;National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicines;Guang anmen Hospital,China Academy of Chinese Medical Sciences;

摘要(Abstract):

采用反相高效液相色谱法,经一系列方法学考察,建立对淫羊藿中朝藿定A、朝藿定B、朝藿定C、淫羊藿苷和宝藿苷Ⅰ5种黄酮类成分进行同步含量测定的方法。采用"一测多评"中药质量评价模式,以淫羊藿苷为内参物,建立朝藿定A、朝藿定B、朝藿定C和宝藿苷Ⅰ与淫羊藿苷间的相对校正因子与相对保留值,最终实现了在同一色谱条件下,仅采用淫羊藿苷1个中药对照品对中药材淫羊藿、淫羊藿饮片和含淫羊藿中成药抗骨增生胶囊中上述5种黄酮类成分的同步质量控制,建立了从药材-饮片-中成药一体的含淫羊藿中药"一测多评"质量评价模式,为单味药QAMS方法在复方中的推广应用起到示范作用。
Through a series of methodology investigations,we established a new method for simultaneous analysis of epimedins A,B,C,icariin and baohuoside I in Epimedii Folium by using high performance liquid chromatography( HPLC). Meanwhile,using Icariin as an internal reference substance to establish the relative correct factors and relative retention values of Epimedins A,B,C and Baohuoside I to Icariin,then using the quantitative analysis of multi-components by single-marker( QAMS) model,the five analytes can be quantitatively determined in Epimedii Folium and its processed products as well as Kanggu Zengsheng capsule only by measuring the content of icariin in the corresponding samples. All these analysis are completed in the same chromatorgraphic conditions. This paper played the part of demonstration role in the popularization and application of QAMS method established in a single herb to the proprietary Chinese medicines containing this herb.

关键词(KeyWords): 一测多评;黄酮;淫羊藿药材和饮片;抗骨增生胶囊;一体化研究
quantitative analysis of multi-components by single-marker(QAMS);flavonoids;Epimedii Folium and its processed products;Kanggu Zengsheng capsule;integrative research

Abstract:

Keywords:

基金项目(Foundation): 2007年中医药行业科技专业项目(200707009);; 国家“重大新药创制”科技重大专项(2009ZX09301-005,2009ZX0930 8-003);; 中药饮片质量保障体系研究项目(201507002)

作者(Author): 冯伟红;李春;张锴镔;杨立新;易红;陈两绵;张永欣;荣立新;王智民;
FENG Wei-hong;LI Chun;ZHANG Kai-bin;YANG Li-xin;YI Hong;CHEN Liang-mian;ZHANG Yong-xin;RONG Li-xin;WANG Zhi-min;Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences;National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicines;Guang anmen Hospital,China Academy of Chinese Medical Sciences;

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