中国中药杂志

2020, v.45(21) 5280-5288

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扎里奴思方水提液在大鼠血浆、胆汁、尿液和粪便中代谢产物鉴定
Identification of metabolites of Zhali Nusi Prescription in rat plasma, bile, urine and feces after oral administration

张婷;牛阳;黄楷迪;卜凡淑;卞晓坤;赵秋龙;郭盛;尚尔鑫;钱大玮;段金廒;
ZHANG Ting;NIU Yang;HUANG Kai-di;BU Fan-shu;BIAN Xiao-kun;ZHAO Qiu-long;GUO Sheng;SHANG Er-xin;QIAN Da-wei;DUAN Jin-ao;Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing University of Chinese Medicine;The Key Laboratory of Ministry of Education of Ningxia Medical University, Ningxia Medical University;Key Laboratory of Chinese Medicinal Resources Recycling Utilization, State Administration of Traditional Chinese Medicine, Nanjing University of Chine

摘要(Abstract):

研究扎里奴思方在大鼠体内的代谢产物。采用超高效液相色谱-线性离子肼-静电轨道肼串联质谱联用方法(UHPLC-LTQ-Orbitrap-MS)对扎里奴思方在大鼠体内的代谢产物进行分析鉴定。采用ACQUITY UPLC BEH T_3色谱柱(2.1 mm×100 mm,1.7μm),以0.1%甲酸水(A)-乙腈(B)为流动相进行梯度洗脱,在电喷雾质谱负离子模式下对生物样品进行分析。建立了大鼠给药后生物样品的分析方法,共鉴定了8个原型成分和36个代谢产物。结果表明,扎里奴思方主要成分在大鼠体内的代谢途径主要包括甲基化、葡萄糖醛酸化、硫酸化等。为进一步探讨扎里奴思方体内过程,阐明其功效物质基础提供依据。
This study was designed to determine the metabolites of Zhali Nusi Prescription(ZLNSP) in rats. The ultra-high performance liquid chromatography-LTQ Orbitrap mass spectrometric(UHPLC-LTQ-Orbitrap-MS) and mass defect filter techniques were applied to analyze the metabolites of ZLNSP in rat plasma, bile, urine and feces. The biological samples were analyzed by ACQUITY UPLC BEH T_3 column(2.1 mm×100 mm,1.7 μm), with 0.1% formic acid water(A)-acetonitrile(B) as mobile phase, and the biological samples were analyzed in negative ion mode by electrospray ionization mass spectrometry(ESI-MS). An analytical method for biological samples of rats was established, and 8 prototype components and 36 metabolites were identified. The results showed that the metabolic pathways of the main components of ZLNSP in rats included methylation, glucuronidation, sulfation and so on. It provi-ded information for the therapeutic effect of ZLNSP in vivo.

关键词(KeyWords): 扎里奴思方;超高效液相色谱;线性离子肼-静电轨道肼串联质谱;代谢产物
Zhali Nusi Prescription;ultra high performance liquid chromatography;LTQ-Orbitrap-MS;metabolite

Abstract:

Keywords:

基金项目(Foundation): 宁夏回族自治区重点研发计划(东西部合作)项目(2017BY084)

作者(Author): 张婷;牛阳;黄楷迪;卜凡淑;卞晓坤;赵秋龙;郭盛;尚尔鑫;钱大玮;段金廒;
ZHANG Ting;NIU Yang;HUANG Kai-di;BU Fan-shu;BIAN Xiao-kun;ZHAO Qiu-long;GUO Sheng;SHANG Er-xin;QIAN Da-wei;DUAN Jin-ao;Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing University of Chinese Medicine;The Key Laboratory of Ministry of Education of Ningxia Medical University, Ningxia Medical University;Key Laboratory of Chinese Medicinal Resources Recycling Utilization, State Administration of Traditional Chinese Medicine, Nanjing University of Chine

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DOI: 10.19540/j.cnki.cjcmm.20200623.201

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