中国中药杂志

2011, v.36(08) 1071-1074

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应用蛋白质组学技术筛选金银花抗流感病毒血清标志物
Screen anti-influenza virus serum marker of Lonicera japonica by proteomics technology

张会敏;宋健;石俊英;
ZHANG Huimin1,SONG Jian2,SHI Junying2(1.Shandong Academy of Traditional Chinese Medicine,Jinan 250014,China;2.Shandong University of Traditional Chinese Medicine,Jinan 250355,China)

摘要(Abstract):

目的:比较不同实验组的小鼠血清蛋白的差异以寻找金银花抗流感病毒候选血清标志物,为阐明金银花抗流感病毒作用的途径及机制提供依据。方法:用双向电泳(2-DE)分离不同实验组的小鼠血清蛋白并比较差异,选取血清中明显差异表达蛋白点,进行质谱鉴定和生物信息学分析。结果:经2-DE找出了不同实验组间较为典型的具明显差异的蛋白质点15个,并通过MALD I-TOF-MS进行了结构鉴定,检索得到其中的12个功能蛋白的成分归属。结论:不同实验组的小鼠血清蛋白之间存在明显差异,通过蛋白质组学方法筛选并鉴定出这些血清标志物,为阐明金银花抗流感病毒作用途径及机制提供依据。
Objective: The candidate anti-influenza virus serum marker of Lonicera japonica was searched by comparing the serum protein discrepancy of experimental groups.It will provide the basis for setting forth the action and mechanism of anti-influenza virus.Method: Two-dimensional electrophoresis(2-DE) was used to separate different experimental groups and the differences in serum protein was compared.The significantly expressed protein spots was selected for mass spectrum identification and bioinformatics analysis.Result: Fifteen identified points of protein have more typical differences between experimental groups.The structures were identified by MALDI-TOF-MS,and 12 functional protein component ownership were retrieved.Conclusion: There was significant difference between the serum protein of experimental groups Proteomic methods can be used to select and identify the serum marker.It is expected to clarify the mechanism of anti-influenza virus of L.japonica

关键词(KeyWords): 金银花;蛋白质组学;流感病毒;标志物
Lonicera japonica;proteomics;influenza virus;marker

Abstract:

Keywords:

基金项目(Foundation): 山东省自然科学基金项目(Y2007C177)

作者(Authors): 张会敏;宋健;石俊英;
ZHANG Huimin1,SONG Jian2,SHI Junying2(1.Shandong Academy of Traditional Chinese Medicine,Jinan 250014,China;2.Shandong University of Traditional Chinese Medicine,Jinan 250355,China)

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