中国中药杂志

2018, v.43(11) 2326-2332

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不同来源引流熊胆粉的化学成分系统分析
Systematical analysis of multiple components in drainage bear bile powder from different sources

王杰;熊爱珍;程绒绒;杨莉;王峥涛;刘绍勇;
WANG Jie;XIONG Ai-zhen;CHENG Rong-rong;YANG Li;WANG Zheng-tao;LIU Shao-yong;The Key Laboratory of Standardization of Chinese Medicines of Ministry of Education,Institute of Chinese Materia Medica,Shanghai University of Traditional Chinese Medicine;Shanghai R&D Centre for Standardization of Chinese Medicines;Shanghai Kai Bao Pharmaceutical Co.,Ltd.;

摘要(Abstract):

熊胆粉中含有胆汁酸、胆红素、蛋白质、氨基酸、微量元素等多种成分,其中胆汁酸类是熊胆粉中含量最高且研究最多的活性成分,而非胆汁酸类成分目前却鲜有报道。因此,该研究对来源为四川、黑龙江、云南的熊胆粉中各化学成分进行系统的研究。通过采用微孔板分光光度计法测定胆红素、蛋白质,利用液相色谱质谱联用技术测定胆汁酸和氨基酸,利用电感耦合等离子体质谱仪测定微量元素。并采用相似度评价软件对不同来源的20批熊胆粉进行系统分析,表明不同来源熊胆粉中胆汁酸类成分相似度高(相似度系数为0.9220.977),不存在明显差异。所测样本中牛磺熊去氧胆酸与牛磺鹅去氧胆酸是熊胆粉中含量最高的成分,为41%0.977),不存在明显差异。所测样本中牛磺熊去氧胆酸与牛磺鹅去氧胆酸是熊胆粉中含量最高的成分,为41%59%,均符合现行卫生部质量标准。二者总含量在来源为黑龙江的熊胆粉中最高,对不同来源的熊胆粉有分类意义。此外,不同来源熊胆粉中胆红素、蛋白质、氨基酸、微量元素含量也存在着一定的差异。该研究发现不同来源熊胆粉主要化学成分较为相似,但胆汁酸、胆红素、蛋白质、氨基酸、微量元素对不同来源的样本有分类意义,该研究为市售熊胆粉的质量评价和控制提供了更加全面科学的依据。
Bear Bile Powder contains bile acids,protein,amino acids,bilirubin and microelements and other compounds. Among them,the bile acids are the most active components. Currently,there are many studies on bile acids,but few reports on other components. Therefore,the purpose of this study is to carry out a systematical analysis of multiple components in drainage Bear Bile Powder from different sources. Bilirubin and protein were quantified by microplate spectrophotometer. The contents of bile acids and amino acids were determined by liquid chromatography coupled with mass spectrometry( LC-MS). The contents of microelements were determined by inductively coupled plasma mass spectrometry( ICP-MS) The result indicated that among 20 batches of bear bile powder from different sources there is high similarity( 0. 922-0. 977). Tauroursodeoxycholic acid( TUDCA) and taurochenodeoxycholic acid( TCDCA) were the two most abundant components. The total contents of them were 41%-59% and met the current standard for quality control of bear bile powder. However,significant differences were found in their contents among samples from different sources. Besides,bilirubin,protein,amino acids and microelements also contributed to the differentiation of samples from different sources. The main components of bear bile powder from the different sources were with satisfactory similarity. But bile acids,bilirubin,protein,amino acids and microelements all contributed to the different among samples. Our present study provided a systematical approach for the better quality control and evaluation of bear bile powder.

关键词(KeyWords): 熊胆粉;胆汁酸;氨基酸;微量元素;胆红素;蛋白质
bear bile power;bile acids;amino acids;microelements;bilirubin;protein

Abstract:

Keywords:

基金项目(Foundation): 国家“重大新药创制”科技重大专项(2017ZX09309006);; 上海市科委技术标准专项(16DZ0501000)

作者(Authors): 王杰;熊爱珍;程绒绒;杨莉;王峥涛;刘绍勇;
WANG Jie;XIONG Ai-zhen;CHENG Rong-rong;YANG Li;WANG Zheng-tao;LIU Shao-yong;The Key Laboratory of Standardization of Chinese Medicines of Ministry of Education,Institute of Chinese Materia Medica,Shanghai University of Traditional Chinese Medicine;Shanghai R&D Centre for Standardization of Chinese Medicines;Shanghai Kai Bao Pharmaceutical Co.,Ltd.;

DOI: 10.19540/j.cnki.cjcmm.20180125.001

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