中国中药杂志

2014, v.39(19) 3836-3840

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金叶子异槲皮苷对MC3T3-E1细胞成骨分化的影响
Effects of isoquercitrin from Craibiodendron yunnanense on osteogenic differentiation of MC3T3-E1 cells

段爱竹;邓旭亮;李蓉涛;
DUAN Ai-zhu;DENG Xu-liang;LI Rong-tao;Faculty of Life Science and Technology,Kunming University of Science and Technology;Department of Geriatric Dentistry,Peking University School and Hospital of Stomatology;

摘要(Abstract):

该研究对从金叶子中分离得到的异槲皮苷的成骨活性进行了系统评价。在1×10-4,1×10-5,1×10-6,1×10-7mol·L-1异槲皮苷浓度作用下检测了MC3T3-E1细胞增殖活力和碱性磷酸酶活性;在异槲皮苷作用的第3天对MC3T3-E1碱性磷酸酶、I型胶原以及转录因子Runx2和Osterix的基因表达水平进行了检测;并通过茜素红染色的方法在第21天对MC3T3-E1进行了胞外基质矿化能力的评价。结果显示,异槲皮苷在1×10-7~1×10-5mol·L-1能促进MC3T3-E1细胞的增殖、分化及矿化能力,上调成骨相关基因的表达,而且该作用呈现出一定的浓度依赖性,在浓度为1×10-6mol·L-1时促进作用最强。在1×10-4mol·L-1时表现出明显的细胞毒性。因此,从金叶子中分离得到的异槲皮苷有一定的成骨活性,这可能是传统中药金叶子治疗骨折的主要药效成分。
Natural products especially flavonoids are being explored for their therapeutic potentials in reducing bone loss and maintaining bone health. The present study is to investigate the effects of isoquercitrin from Craibiodendron yunnanense with different concentrations at 1 × 10- 4,1 × 10- 5,1 × 10- 6,1 × 10- 7mol·L- 1on proliferation,differentiation and mineralization of MC3T3-E1.Cell proliferation was assessed by CCK-8 kit at 1,3,5 and 7 days of culture. Alkaline phosphatase( ALP) activity were performed qualitatively and quantitatively on day 7,and alizarin red S staining was employed to access the mineralization of cells on day 21. The osteogenic markers ALP,collagen type I( COL 1A1),runt-related transcription factor 2( Runx-2) and Osterix were detected to analysis early osteogenic differentiation of cells on day 3 by RT-PCR. The results showed that isoquercitrin had a dose-dependent effect on the proliferation,osteogenic differentiation,mineralization and gene expression of MC3T3-E1 in the range from 1 × 10- 7to 1 × 10- 5mol·L- 1. At concentrations above 1 × 10- 4mol·L- 1isoquercitrin showed cytotoxicity,while 1 × 10- 6mol·L- 1is the optimal concentration of isoquercitrin to improve the osteoblastic activity. All these results implied that isoquercitrin might be the major composition of traditional chinese medicine C. yunnanense to treat bone fractures.

关键词(KeyWords): 异槲皮苷;MC3T3-E1细胞;增殖;成骨分化
isoquercitrin;MC3T3-E1 cell;proliferation;osteogenic differentiation

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基金项目(Foundation): 国家自然科学基金项目(21262021)

作者(Author): 段爱竹;邓旭亮;李蓉涛;
DUAN Ai-zhu;DENG Xu-liang;LI Rong-tao;Faculty of Life Science and Technology,Kunming University of Science and Technology;Department of Geriatric Dentistry,Peking University School and Hospital of Stomatology;

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