中国中药杂志

2017, v.42(13) 2453-2459

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人参皂苷生物合成基因组织表达特性的研究
Characterization of tissue expression of ginsenoside biosynthetic gene expression in Panax ginseng

刘娟;纪瑞锋;陈同;袁媛;郭娟;王英平;高文远;黄璐琦;
LIU Juan;JI Rui-feng;CHEN Tong;YUAN Yuan;GUO Juan;WANG Ying-ping;GAO Wen-yuan;HUANG Lu-qi;State Key Laboratory of Dao-di Herbs Breeding Base,National Resource Center for Chinese Materia Medica,China Academy of Chinese Medical Sciences;Institute of Special Animal and Plant,Chinese Academy of Agricultural Sciences;School of Pharmaceutical Science and Technology,Tianjin University;

摘要(Abstract):

采用生物信息学及实时荧光定量方法对10个人参皂苷生物合成关键酶基因的组织表达特性进行研究。运用转录组热图聚类方法分析了四年生吉林人参14个不同部位人参皂苷生物合成基因的表达;运用实时荧光定量PCR的方法测定人参组培苗、不定根中人参皂苷生物合成基因的表达;运用Pearson相关对这10个人参皂苷生物合成关键酶基因表达特性进行分析。结果表明,β-AS,CYP716A52v2在吉林人参及组培苗的根中表达高,与Ro分布相一致;与达玛烷型人参皂苷合成相关的CYP716A47,CYP716A53v2表达呈显著正相关,从分子水平证明了人参皂苷化学成分分布的差异主要由转运引起。
The study is aimed to characterize the tissue expression of 10 key ginsenoside biosynthetic genes using bioinformatics method and real-time quantitative PCR. Heatmap and cluster analysis of 10 ginsenoside biosynthetic genes were performed in four-yearold Jilin ginseng. Using real-time quantitative PCR,the expression correlation of 10 key genes involved in ginsenoside biosynthesis was analyzed in different organs of four-year-old Jilin ginseng including,tissue culture seedling and adventitious root. Pearson correlation was used to analyze the relation between those 10 key genes involved in ginsenoside biosynthesis. The results showed that β-AS and CYP716A52v2 were expressed highly in root of Jilin ginseng and ginseng culture seedling,which was consistent with Ro distribution. In addition,CYP716A53v2 and CYP716A47 which involved in dammarane type ginsenoside biosynthesis were positively correlated,which revealed that the difference of ginsenoside distribution was caused by transport system.

关键词(KeyWords): 人参;人参皂苷生物合成基因;组织表达
ginseng;ginsenoside biosynthetic genes;tissue expression

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(81603235);; 国家杰出青年科学基金项目(81325023);; 中央本级重大增减支项目(2060302);; 中国中医科学院中药资源中心自主选题研究项目(ZZ2016006)

作者(Author): 刘娟;纪瑞锋;陈同;袁媛;郭娟;王英平;高文远;黄璐琦;
LIU Juan;JI Rui-feng;CHEN Tong;YUAN Yuan;GUO Juan;WANG Ying-ping;GAO Wen-yuan;HUANG Lu-qi;State Key Laboratory of Dao-di Herbs Breeding Base,National Resource Center for Chinese Materia Medica,China Academy of Chinese Medical Sciences;Institute of Special Animal and Plant,Chinese Academy of Agricultural Sciences;School of Pharmaceutical Science and Technology,Tianjin University;

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