中国中药杂志

2011, v.36(07) 871-873

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HPLC法测定茱萸和胃止痛胶囊中吴茱萸碱和吴茱萸次碱的含量
Determination of evodiamine and rutacarpine in Zhuyu Hewei Zhitong capsules by HPLC

麻秀萍;李江;刘英波;
MA Xiuping,LI Jiang,LIU Yingbo(Guiyang College of Traditional Chinese Medicine,Guiyang 550002,China)

摘要(Abstract):

目的:建立高效液相色谱法测定中药新药茱萸和胃止痛胶囊中吴茱萸碱和吴茱萸次碱的含量。方法:色谱柱为Ec lipse XDB-C18(4.6 mm×150 mm,5μm),流动相为乙腈-水(45∶55),柱温30℃,检测波长为250 nm。结果:吴茱萸碱和吴茱萸次碱的线性范围分别为0.051 00.560,0.050 80.560,0.050 80.559μg;平均回收率分别为97.5%(RSD 1.0%),98.7%(RSD1.5%)。结论:该法专属性强,准确稳定,重复现性好,可作为茱萸和胃止痛胶囊的质量控制方法。
Objective: To develop a HPLC method for the determination of evodiamine and rutacarpine in Zhuyu Hewei Zhitong capsules.Method: The separation was performed at 30 ℃ on an Eclipse XDB-C18 column(4.6 mm×150 mm,5 μm) eluted with the mobile phase consisted of acetonitrile-water(45∶55).The detection wavelength was 250 nm.Result: The standard curve for evodiamine is linear in the range of 0.051 0-0.560 μg,the average recovery was 97.5%(RSD 1.0%).The standard curve for rutacarpine is linear in the range of 0.050 8-0.559 μg,the average recovery was 98.7%(RSD 1.5%).Conclusion: The method is specific,accurate and stable.It can be used for detemination of evodiamine and rutacarpine in Zhuyu Hewei Zhitong capsules.

关键词(KeyWords): 茱萸和胃止痛胶囊;高效液相色谱法;吴茱萸碱;吴茱萸次碱
Zhuyu Hewei Zhitong capsules;HPLC;evodiamine;rutacarpine

Abstract:

Keywords:

基金项目(Foundation): 贵州省中药现代化项目(黔科合农字〔2006〕5020号)

作者(Authors): 麻秀萍;李江;刘英波;
MA Xiuping,LI Jiang,LIU Yingbo(Guiyang College of Traditional Chinese Medicine,Guiyang 550002,China)

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