中国中药杂志

2012, v.37(22) 3381-3385

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白术遗传多样性ISSR分析
Genetic diversity of Atractylodes macrocephala by ISSR

孙靓;郑玉光;袁媛;卜璟;王雪;蒋超;陈敏;
SUN Liang1,2,ZHENG Yu-guang3,YUAN Yuan1,BU Jing1,WANG Xue1,JIANG Chao1,CHEN Min1(1.Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China; 2.Jiangxi University of Traditional Chinese Medicine,Nanchang 330006,China; 3.Hei Medical University,Shijiazhuang 050091,China)

摘要(Abstract):

目的:对白术12个栽培居群及3个半野生居群的遗传多样性进行分析。方法:应用ISSR分子标记技术研究浙江、安徽、河北等省15个居群356个样品的遗传多样性;采用POPGEN32进行数据分析,UPGMA绘制聚类图。结果:13条ISSR引物共检测到102个清晰的扩增位点,多态性位点94个,多态位点百分率为92.16%;Nei's基因多样性指数(He)为0.406 5,Shannon多样性指数(I)为0.590 3,基因分化系数(Gst)为0.202 5。居群间的遗传相似系数为0.690 7~0.960 5,平均为0.825 6。聚类分析可知,居群间并无明显分类。结论:白术具有较高的遗传多样性,遗传分化水平低,无明显地域性差异及品种差异。这与白术目前栽培及种质流通现状呈正相关。
Objective: To assess the genetic diversity of Atractylodes macrocephala in 12 cultivated populations and 3 wild populations.Method: The molecular marker technique ISSR was used to investigate the genetic diversity of 365 samples from 15 populations in Zhejiang,Anhui and Hebei Provinces.Data was analyzed by POPGEN 32,and a cluster diagram was presented by UPGMA.Result: One hundred and two amplified fragments were obtained using 13 ISSR primers.94 polymorphic loci were identified as 92.16%.Nei′s genetic diversity index(He) was 0.406 5,Shannon diversity index(I) was 0.590 3,and the coefficient of gene differentiation(Gst) was 0.202 5.The genetic similarity coefficient among the populations ranged from 0.690 7 to 0.960 5 in an average of 0.825 6.No significant difference was found among the populations based on the clustering analysis.Conclusion: There is a low level of genetic differentiation in higher genetic diversity of the A.macrocephala populations.No obvious regional or species diversities were identified.This is consistent with current status of cultivation and gerplasm circulation in A.macrocephala.

关键词(KeyWords): 白术;遗传多样性;ISSR
Atraetylodes maerocephala;genetic diversity;ISSR

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基金项目(Foundation): 国家“重大新药创制”科技重大专项(2009ZX09308-002)

作者(Author): 孙靓;郑玉光;袁媛;卜璟;王雪;蒋超;陈敏;
SUN Liang1,2,ZHENG Yu-guang3,YUAN Yuan1,BU Jing1,WANG Xue1,JIANG Chao1,CHEN Min1(1.Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China; 2.Jiangxi University of Traditional Chinese Medicine,Nanchang 330006,China; 3.Hei Medical University,Shijiazhuang 050091,China)

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