中国中药杂志

2013, v.38(17) 2854-2858

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黄芩苷对LPS诱导肠道损伤的保护作用研究
Protective effect of baicalin against LPS-induced intestinal injury

吴琼;叶华;朱宇珍;郭蒙;荷香溪;郑学宝;
WU Qiong;YE Hua;ZHU Yu-zhen;GUO Meng;HE Xiang-xi;ZHENG Xue-bao;Teaching and Research Room of Pharmacology,Guangdong Medical College;Guangdong Key Laboratory for Research and Development of Natural Drugs,Guangdong Medical College;

摘要(Abstract):

目的:探讨黄芩苷对内毒素-脂多糖(LPS)引起的肠损伤的肠黏膜保护作用及黄芩苷对脓毒血症小鼠回、结肠黏膜抗氧化损伤的保护作用。方法:健康雄性BALB/c小鼠50只随机分为5组:正常对照组、模型组、黄芩苷高、中、低剂量组。分别灌胃双蒸水、黄芩苷100,50,25 mg·kg-1,每日1次,连续3 d,于第3天灌胃后1 h,腹腔注射生理盐水或LPS(17 mg·kg-1),于腹腔注射LPS后20 h处死小鼠,取回、结肠组织。观察各组小鼠生活、精神状态,记录各组小鼠死亡率,取材后测量结肠长度;光镜下根据Chiu肠黏膜损伤评分方法对肠组织进行分级,以HE染色方法观察肠组织病理学变化;采用紫外分光光度法检测肠组织匀浆液总抗氧化能力(T-AOC)、超氧化物歧化酶(T-SOD)、谷胱甘肽过氧化物酶(GSH-PX);免疫组化染色方法分析各组肠组织PCNA的表达。结果:小鼠腹腔注射LPS后,出现死亡现象,黄芩苷给药组死亡百分率明显低于模型组。黄芩苷中剂量组小鼠结肠长度较模型组显著增加(P<0.05);肠黏膜损伤程度较模型组显著降低,回、结肠的高剂量组损伤较模型组显著降低(P<0.05),回、结肠的中、低剂量组损伤较模型组显著降低(P<0.000 1);中剂量回肠匀浆液T-AOC,T-SOD,GSH-PX较模型组显著增加(P<0.05);黄芩苷组较模型组PCNA表达无显著性差异。结论:黄芩苷有保护肠上皮细胞免受氧自由基损伤的能力,其通过改善肠黏膜结构与功能减轻LPS引起的肠道损伤。
Objective: To investigate the protective effect of baicalin on the intestinal mucosal injury caused by endotoxin-lipopolysaccharide(LPS) and the anti-oxidative injury in colonic and ileal mucosa of rats with septicopyemia.Method: Fifty healthy male BALB / c mice were randomly divided into 5 groups: the normal control group,the model group,and baicalin high-dose,mediumdose and low-dose groups.They were orally administered with double distilled water,100 mg·kg-1of baicalin,50 mg·kg-1of baicalin,and 25 mg·kg-1of baicalin respectively for three days,once a day.1 h after the oral administration on 3 d,they were intraperitoneally injected with normal saline or LPS(17 mg·kg-1).At 20 h after the injection of LPS,all of the mice were sacrificed,and their colonic and ileal tissues were collected.The mental status,life state and death rate of mice in each group were observed,and the lengths of colonic were measured.Chiu's scoring method was used to assess the intestinal mucosal injury.Histopathological changes of intestinal tissues were tested by HE staining.The ultraviolet spectrophotometry was used to detect total antioxidant capacity(T-AOC),superoxide dismutase(T-SOD),and glutathione peroxidase(GSH-PX) of intestinal homogenate.The immunohistochemical method was used to analyze the expression of PCNA in intestinal tissues of each group.Result: The death of mice was observed after the intraperitoneal injection of LPS.The death rates of baicalin groups were remarkably lower than the death rate of the model group.The colons in the medium-dose baicalin group were much longer than that in the model group(P < 0.05),with a much lower intestinal mucosa injury degree than the model group.Colonic and ileal injuries in the high-dose baicalin group significantly(P < 0.05).Colonic and ileal injuries in the medium-dose baicalin group and the low-dose baicalin group significantly reduced compare with the model group(P < 0.000 1).The medium-dose baicalin group showed no significant increase in homogenate's T-AOC,T-SOD and GSH-PX compare with the model group(P < 0.05).There was no significant difference between baicalin groups and the model group in PCNA.Conclusion: Baicalin can protect intestinal epithelial cells suffering from injury from oxygen radicals,and relieve the intestinal injury caused by LPS by improving the intestinal mucosa structure and functions.

关键词(KeyWords): 脓毒症;黄芩苷;肠保护
sepsis;baicalin;intestinal protection

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(30772701);; 广东省科技计划项目(2011B03170065);; 广东省建设中医药强省项目(20112144);; 湛江市科技攻关项目(2009C3104003)

作者(Authors): 吴琼;叶华;朱宇珍;郭蒙;荷香溪;郑学宝;
WU Qiong;YE Hua;ZHU Yu-zhen;GUO Meng;HE Xiang-xi;ZHENG Xue-bao;Teaching and Research Room of Pharmacology,Guangdong Medical College;Guangdong Key Laboratory for Research and Development of Natural Drugs,Guangdong Medical College;

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