李继昌,王伟明,张洪娟,董龙
LI Ji-chang~1,2 , WANG Wei-ming~1 , ZHANG Hong-juan~1 , DONG Long~1 (1. Heilongjiang Academy of Traditional Chinese Medicine Research Institute, Harbin 150036, China; 2. Animal Medicine College, Northeast Agricultural University, Harbin 150030, China) [

• 1:黑龙江省中医研究院中药研究所
• 2:黑龙江省中医研究院中药研究所
• 3:黑龙江省中医研究院中药研究所
• 4:黑龙江省中医研究院中药研究所 黑龙江哈尔滨150036

摘要(Abstract)：

目的研究呼畅清肺浓缩丸提高肺炎支原体感染大鼠免疫功能的作用及机制。方法采用滴鼻法建立大鼠肺炎支原体感染模型,通过放射免疫法、速率散射比浊法和流式细胞仪技术研究呼畅清肺浓缩丸对模型大鼠体液免疫和细胞免疫功能的影响。结果与模型组比较,呼畅清肺浓缩丸能明显提高感染大鼠血清中IgG,IgM,IL2和IL6水平,降低TNFα和C3补体含量,并使CD+4/CD+8比值增高。结论呼畅清肺浓缩丸能够明显改善支原体感染大鼠体液免疫和细胞免疫功能低下的状态,具有提高机体抗病原体感染的免疫作用。
Objective: To study the effects and mechanism of Huchang Qingfei pellets on immune function in rats infected with mycoplasma pneumoniae. Method: A rat model of mycoplasmal pneumonia (MP) was developed in repeated intranasal infectious routes and then the humoral and cellular immunocompetences were detected by radioimmunoassay,immune-turbidimetry and flow cytometry. Result: The levels of serum IgG,IgM and IL-2,IL-6 were enhinced obviously, the complement C_~3 and TNF-α were decreased and the ratio of CD+_4/CD+_8 was improved significantly in the Huchang groups as compared with MP model group. Conclusion: Huchang Qingfei pellets can reinforce immune function via preventing both cellular and humoral immunity from depression in the rats with MP. [

关键词(KeyWords)： 中药复方制剂;肺炎支原体;细胞免疫;体液免疫
Chinese traditional herbs; mycoplasma pneumoniae; cellular immunity; humoral immunity

Abstract:

Keywords:

基金项目(Foundation): 国家中医药管理局新药开发专项基金(DIX055B)

作者(Author): 李继昌,王伟明,张洪娟,董龙
LI Ji-chang~1,2 , WANG Wei-ming~1 , ZHANG Hong-juan~1 , DONG Long~1 (1. Heilongjiang Academy of Traditional Chinese Medicine Research Institute, Harbin 150036, China; 2. Animal Medicine College, Northeast Agricultural University, Harbin 150030, China) [

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DOI:

参考文献(References)：

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