中国中药杂志

2011, v.36(08) 1087-1090

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小柴胡汤在Caco-2细胞模型的吸收特性和转运机制研究
Study on transportation of Xiaochaihu Tang in Caco-2 cell model

鄢良春;刘青春;赵军宁;毕岳琦;易进海;
YAN Liangchun1,LIU Qingchun1,2,ZHAO Junning1,BI Yueqi1,YI Jinhai1(1.Institute of Pharmacology and Toxicology,Key Laboratory of Biological Evaluation of Traditional Chinese Medicine Quality of the Administration of Traditional Chinese Medicine,Sichuan Provincial Key Laboratory of the Quality and Innovation Research of Chinese Materia Medica,Sichuan Academy of Chinese Medicine Sciences,Chengdu 610041,China;2.West China-Frontier Pharma Tech Co.,Ltd.,Chengdu 610041,China)

摘要(Abstract):

目的:研究小柴胡汤在Caco-2细胞模型的吸收特性和转运机制。方法:利用MTT实验筛选小柴胡汤在Caco-2细胞中的安全浓度,用Caco-2细胞模型研究小柴胡汤的双向转运情况,采用高效液相色谱法进行含量测定,考察时间对小柴胡汤吸收的影响。结果:汉黄芩苷(wogonoside,WGS)和汉黄芩素(wogonin,WGN)由AP侧到BL侧的Papp分别为(1.23±0.09)×10-6,(1.07±0.89)×10-5,由BL侧到AP侧的Papp分别为(2.12±0.19)×10-6,(7.12±1.02)×10-6,二者的转运率随着时间的增加而增加,WGS和WGN的PappAP→BL/PappBL→AP分别为0.58,1.49。黄芩苷、黄芩素和甘草酸不能透过Caco-2细胞单层。结论:WGS和WGN在Caco-2细胞单层模型中的转运途径可能为被动转运。
Objective: To study the transportation of Xiaochaihu Tang in Caco-2 cell model.Method:The safety concentration of Xiaochaihu Tang in Caco-2 cells was determined by MTT assay.Then the Caco-2 cell model was used to investigate the bi-directional transportation of Xiaochaihu Tang.The multicomponents of Xiaochaihu Tang and the influence of time were measured by high performance liquid chromatography(HPLC).Result: The Papp values of wogonoside and wogonin were(1.23±0.09)×10-6,(1.07±0.89)×10-5 cm·s-1 from the AP side to BL side,and(2.12±0.19)×10-6and(7.12±1.02)×10-6 cm·s-1 from the BL side to AP side,respectively.The PappAP→BL/Papp BL→APratio of wogonoside and wogonin were 0.58 and 1.49,respectively.Baicalin,baicalein and glycyrrhizic acid could not permeate the Caco-2 cell model.Conclusion: The transportation of wogonoside and wogonin in Caco-2 cell model may be a passive transportation.

关键词(KeyWords): 小柴胡汤;Caco-2细胞模型;转运
Xiaochaihu Tang;Caco-2 cell model;transportation

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基金项目(Foundation): 国家自然科学基金项目(30672651)

作者(Author): 鄢良春;刘青春;赵军宁;毕岳琦;易进海;
YAN Liangchun1,LIU Qingchun1,2,ZHAO Junning1,BI Yueqi1,YI Jinhai1(1.Institute of Pharmacology and Toxicology,Key Laboratory of Biological Evaluation of Traditional Chinese Medicine Quality of the Administration of Traditional Chinese Medicine,Sichuan Provincial Key Laboratory of the Quality and Innovation Research of Chinese Materia Medica,Sichuan Academy of Chinese Medicine Sciences,Chengdu 610041,China;2.West China-Frontier Pharma Tech Co.,Ltd.,Chengdu 610041,China)

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