中国中药杂志

2009, v.34(09) 1117-1120

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白芷酪氨酸酶抑制成分研究
Tyrosinase inhibitor from Angelica dahurica roots

朴香兰;
PIAO Xianglan(Institute of Chinese Minority Traditional Medicine,Minzu University of China,Beijing 100081,China)

摘要(Abstract):

目的:热处理白芷Angelica dahurica,并寻找其中的酪氨酸酶抑制成分。方法:采用生物活性-高效液相色谱跟踪方法,以及硅胶柱色谱法,分离、鉴定热处理白芷中酪氨酸酶抑制成分。结果:热处理白芷提取物比原药显示出更强的酪氨酸酶抑制作用,并从其有效部位中分离得到8-羟基-5-甲氧基补骨脂素(IC50=0.0086 mmol.L-1),比阳性对照曲酸(kojic acid)(IC50=0.0907 mmol.L-1)对酪氨酸酶抑制活性更强。结论:热处理明显提高了白芷酪氨酸酶抑制活性,8-羟基-5-甲氧基补骨脂素为活性成分。
Objective: To isolate and identify the tyrosinase inhibitory constituent from Angelica dahurica.Method: A tyrosinase inhibitory constituent was isolated by silica gel column chromatography combined with a bioassay-linked HPLC method.Result: An extract of heat-processed A.dahurica roots showed more potent tyrosinase inhibitory activity than that of the crude drug.Tyrosinase inhibitory furanocoumarin,8-hydroxy-5-methoxypsoralen,was isolated.It express stronger tyrosinase inhibition than the known tyrosinase inhibitor,kojic acid(IC50=0.090 7 mmol·L-1) with IC50 value of 0.008 6 mmol·L-1.Therefore,it is contributive to the increased tyrosinase inhibitory activity of the heat-processed A.dahurica.Conclusion: The active constituent was 8-hydroxy-5-methoxypsoralen from the heat-processed A.dahurica.

关键词(KeyWords): 白芷;酪氨酸酶抑制活性;8-羟基-5-甲氧基补骨脂素
Angelica dahurica;tyrosinase inhibitory activity;8-hydroxy-5-methoxypsoralen

Abstract:

Keywords:

基金项目(Foundation): 留学回国人员科研启动基金项目(教外司留[2007]1108)

作者(Authors): 朴香兰;
PIAO Xianglan(Institute of Chinese Minority Traditional Medicine,Minzu University of China,Beijing 100081,China)

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