中国中药杂志

2011, v.36(13) 1798-1802

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芒果苷对慢性炎症MAPK信号通路的影响
Effects of mangiferin on MAPK signaling pathway in chronic inflammation

卫智权;阎莉;邓家刚;邓静;
WEI Zhiquan 1,YAN Li2,DENG Jiagang2,DENG Jing3(1.Guangxi Medical University,Nanning 530021,China;2.Guangxi Traditional Chinese Medicine University,Nanning 530001,China;3.Dana-Farber Cancer Institute,Harvard Medical School,Boston,Massachusetts 02115,USA)

摘要(Abstract):

目的:探讨芒果苷调控MAPK信号通路而抑制脂多糖(LPS)诱导慢性炎症的机制。方法:60只SD大鼠随机分为正常组、模型组、阳性药泼尼松5 mg.kg-1.d-1组与芒果苷200,100,50 mg.kg-1.d-1组。以LPS间断尾静脉注射建立慢性炎症模型,进行大鼠全血白细胞计数,ELISA测定血清肿瘤坏死因子(TNF-α)、白细胞介素6(IL-6)、可溶性细胞间黏附分子1(sICAM-1);RT-PCR检测白细胞MAPK信号通路p38,ERK,JNK基因表达。结果:与模型组比较,芒果苷200 mg.kg-1组全血白细胞总数与血清TNF-α,IL-6,sICAM-1水平明显降低(P<0.05),白细胞ERK,JNK基因表达下调(P<0.05),p38基因表达无统计学差异。结论:芒果苷显著抑制LPS诱导慢性炎症的作用机制与其下调MAPK信号通路中ERK,JNK基因表达而降低炎症因子水平有关。
Objective: To investigate mechanism of inhibition on the lipopolysaccharide induced chronic inflammation of mangiferin by the regulation of mitogen-activated protein kinase(MAPK) signaling pathway.Method: Sixty SD rats were randomly divided into normal control,model control,positive drug control(prednisone,5 mg·kg-1·d-1) and mangiferin(200,100,50 mg·kg-1·d-1) group.The chronic inflammation models were established by intermittent injection of lipopolysaccharide via the tail vein.The leucocyte count was measured.The levels of serum tumor necrosis factor alpha(TNF-α),interleukin-6(IL-6) and soluble intercellular adhesion molecule 1(sICAM-1) were detected by enzyme-linked immunosorbent assay(ELISA).The reverse transcription-polymerase chain reaction(RT-PCR) was applied to evaluate the expressions of p38,ERK,JNK gene of leucocyte in MAPK signaling pathway.Result: Compared with the model control,not only the leucocyte count and the level of serum TNF-α,IL-6,sICAM-1 but also the expressions of ERK,JNK gene of leukocyte were markedly reduced in mangiferin(200 mg·kg-1·d-1) group(P<0.05).However,there was no statistics significance for the expression of p38 gene between the model control and the mangiferin(200 mg·kg-1·d-1) group.Conclusion: As a possible mechanism,the regulation of mangiferin on the expressions of ERK,JNK gene of leukocyte in MAPK signaling pathway was involved in its great inhibition on the chronic inflammation induced by lipopolysaccharide.

关键词(KeyWords): 芒果苷;慢性炎症;MAPK信号通路;基因表达
mangiferin;chronic inflammation;MAPK signaling pathway;gene expression

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(30973916);; 广西科技厅自治区重点实验室培育项目(09320005)

作者(Authors): 卫智权;阎莉;邓家刚;邓静;
WEI Zhiquan 1,YAN Li2,DENG Jiagang2,DENG Jing3(1.Guangxi Medical University,Nanning 530021,China;2.Guangxi Traditional Chinese Medicine University,Nanning 530001,China;3.Dana-Farber Cancer Institute,Harvard Medical School,Boston,Massachusetts 02115,USA)

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