中国中药杂志

2021, v.46(05) 1134-1140

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UPLC-MS/MS分析薄荷配方颗粒与传统饮片中非挥发性成分的相关性
Correlation study on non-volatile ingredients of Menthae Haplocalycis Herba formula granules and traditional herbal pieces by UPLC-MS/MS

甄亚钦;田伟;支雅婧;王梦;李志远;牛丽颖;
ZHEN Ya-qin;TIAN Wei;ZHI Ya-jing;WANG Meng;LI Zhi-yuan;NIU Li-ying;Hebei University of Chinese Medicine;Hebei Traditional Chinese Medicine (TCM) Formula Granule Innovation Center;Hebei TCM Quality Evaluation & Standardization Engineering Research Center;SCIEX China;

摘要(Abstract):

建立UPLC-MS/MS同时测定薄荷配方颗粒和传统饮片中新绿原酸、绿原酸、隐绿原酸、咖啡酸、异绿原酸A、迷迭香酸、阿魏酸、芦丁、木犀草苷、异槲皮苷、橙皮苷、香叶木苷、香叶木素、木犀草素、金合欢素和蒙花苷16种非挥发性化学成分的方法,并分析薄荷配方颗粒与传统饮片中非挥发性成分的相关性。采用Shim-pack GIST C_(18)色谱柱(2.1 mm×100 mm, 2μm),流动相为乙腈-0.1%甲酸水溶液,梯度洗脱,流速为0.4 mL·min~(-1),柱温35℃。采用电喷雾离子源(ESI),多反应监测模式(MRM)进行定量分析。结果16种化学成分的线性关系、分离度、重复性及回收率等均符合要求。薄荷饮片中16种非挥发性成分在薄荷配方颗粒中均可得到追踪,不同厂家薄荷配方颗粒和不同产地薄荷饮片中16种化学成分存在一定差异。建立的UPLC-MS/MS分析方法简便、快速、准确,可用于薄荷配方颗粒和传统饮片中非挥发性成分的质量控制。
To establish the method for determining non-volatile ingredients of neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, caffeic acid, isochlorogenic acid A, rosmarinic acid, ferulic acid, rutin, luteoloside, isoquercitrin, hesperidin, diosmin, diosmetin, luteolin, acacetin and linarin in Menthae Haplocalycis Herba formula granules and traditional herbal pieces by UPLC-MS/MS, and analyze the correlation of non-volatile ingredients in Menthae Haplocalycis Herba formula granules and traditional herbal pieces. Shim-pack GIST C_(18) column(2.1 mm×100 mm, 2 μm) was adopted with acetonitrile-0.1% formic acid aqueous solution as the mobile phase for gradient elution at the flow rate of 0.4 mL·min~(-1). The column temperature was set at 35 ℃. The quantitative analysis was performed using the electrospray ionization source and the multiple reaction monitoring. The linear relationship, resolution, repeatability and recovery of the 16 chemical components all met the requirements. The 16 non-volatile ingredients in traditional herbal pieces of Menthae Haplocalycis Herba could be tracked in formula granules. There were certain differences of the 16 chemical components among Menthae Haplocalycis Herba formula granules of different manufacturers and traditional herbal pieces of different producing areas. The UPLC-MS/MS method was simple, rapid and accurate, and could be used for the quality control of non-volatile ingredients in Menthae Haplocalycis Herba formula granules and traditional herbal pieces.

关键词(KeyWords): 薄荷;传统饮片;配方颗粒;液质联用;含量测定;相关性
Menthae Haplocalycis Herba;traditional herbal pieces;formula granules;UPLC-MS/MS;content determination;correlation

Abstract:

Keywords:

基金项目(Foundation): 河北省自然科学基金项目(H2019423050);; 河北省中医药管理局科研计划项目(2017015)

作者(Author): 甄亚钦;田伟;支雅婧;王梦;李志远;牛丽颖;
ZHEN Ya-qin;TIAN Wei;ZHI Ya-jing;WANG Meng;LI Zhi-yuan;NIU Li-ying;Hebei University of Chinese Medicine;Hebei Traditional Chinese Medicine (TCM) Formula Granule Innovation Center;Hebei TCM Quality Evaluation & Standardization Engineering Research Center;SCIEX China;

Email:

DOI: 10.19540/j.cnki.cjcmm.20201221.301

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