中国中药杂志

2006, (13) 1047-1049

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荭草药材的质量研究
Study on quality for Herba Polygoni Orientalis

王爱民;王永林;刘丽娜;李勇军;
WANG Ai-min,WANG Yong-lin,LIU Li-na,LI Yong-jun(School of Pharmacy,Guiyang Medical Colledge,Guiyang 550004,China)

摘要(Abstract):

目的:建立荭草药材的定性、定量检测方法。方法:采用TLC鉴定荭草药材中荭草素、异荭草素、原儿茶酸和没食子酸,HPLC测定荭草中荭草素、异荭草素的质量分数。结果:采用Diamonsil C18色谱柱,以乙腈-0.1%磷酸溶液(18∶82)为流动相,可使异荭草素、荭草素达到基线分离,异荭草素进样量在0.075~0.90μg、荭草素在0.041~0.49μg呈良好的线性关系,异荭草素和荭草素的平均回收率分别为98.8%,99.5%,RSD分别为2.1%,1.7%(n=9)。结论:本方法可以作为荭草药材的定性、定量检测方法。
Objective: To establish the qualitative and quantitative detective methods of Herba Polygoni Orientalis.Method: Isorientin,orientin,protocatechuic acid and gallic acid in Herba Polygoni Orientalis were identified by TLC.The contents of isorientin and orientin in Herba Polygoni Orientalis were determined by HPLC.Result: Isorientin,orientin,protocatechuic acid and gallic acid could be identified by TLC.Isorientin and orientin were well separated with Diamonsil C_(18) column and acetonitrile-0.1% phosphoric acid(18∶82) as mobile phase.The linear range of isorientin was 0.075-0.90 μg.The average recovery of isorientin was 98.8% and RSD was 2.1%.The linear range of orientin was 0.041-0.49 μg.The average recovery of orientin was 98.8% and RSD was 2.1%.Conclusion: The methods can be used for qualitative identification and quantitation determination of Herba Polygoni Orientalis.

关键词(KeyWords): 荭草;异荭草素;荭草素;原儿茶酸;没食子酸;薄层色谱;高效液相色谱
Herba Polygoni Orientalis;isorientin;orientin;protocatechuic acid;glllic acid;TLC;HPLC

Abstract:

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基金项目(Foundation): 贵州省中药现代化科技产业研究开发专项基金项目[黔科合中药专字(2003)12号、黔科合中药专字(2004)03号]

作者(Author): 王爱民;王永林;刘丽娜;李勇军;
WANG Ai-min,WANG Yong-lin,LIU Li-na,LI Yong-jun(School of Pharmacy,Guiyang Medical Colledge,Guiyang 550004,China)

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